The study aimed to investigate the role of exosomes derived from rat bone marrow mesenchymal stem cells (rBMSCs) in acute soft tissue injury and its related mechanisms. Exosomes were isolated from rBMSCs and characterized by Nanosight NS300 particle size analyser (NTA), transmission electron microscopy (TEM), and western blot. Twenty four rats were randomly divided into four groups (n = 6): control group, strike group, rBMSCs group, and rBMSCs‐exo group. Haematoxylin‐eosin (HE) staining was used to observe the morphology. Real‐time quantification PCR (RT‐qPCR) and western blot were used to analyse the expression of IL‐1A, IL‐12A, COL11A1, COL4A4, and Wnt4. NTA, TEM and western blot results showed that exosomes isolated from rBMSCs were cup‐shaped morphology with a size of about 100 nm. HE staining showed that there was severe soft tissue inflammation in strike group, and the symptoms were alleviated after rBMSCs and rBMSCs‐exo treatment. RT‐qPCR and western blot indicated that in the strike group, the expression levels of IL‐1A and IL‐12A were significantly increased, and their expressions were decreased markedly by exosomes treatment. In addition, after treatment, the expression levels of COL11A1 and Wnt4 were up‐regulated, while the expression of COL4A4 was down‐regulated. Exosomes isolated from rBMSCs could improve acute soft tissue injury, and may be used as a new therapeutic strategy acute soft tissue injury.

中文翻译：

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间充质干细胞衍生的外来体作为急性软组织损伤的新治疗策略。

这项研究旨在研究大鼠骨髓间充质干细胞（rBMSCs）衍生的外来体在急性软组织损伤中的作用及其相关机制。从rBMSCs中分离出外泌体，并通过Nanosight NS300粒度分析仪（NTA），透射电子显微镜（TEM）和Western blot进行了表征。24只大鼠随机分为4组（n = 6）：对照组，中风组，rBMSCs组和rBMSCs-exo组。苏木精-伊红（HE）染色用于观察形态。实时定量PCR（RT-qPCR）和Western印迹用于分析IL-1A，IL-12A，COL11A1，COL4A4和Wnt4的表达。NTA，TEM和Western blot结果表明，从rBMSCs分离出的外泌体呈杯状形态，大小约为100 nm。HE染色显示，罢工组严重软组织炎症，经rBMSCs和rBMSCs-exo处理后症状减轻。RT-qPCR和Western blot结果显示，在外来体组中，卒中组IL-1A和IL-12A的表达水平显着升高，而其表达则明显降低。另外，治疗后，COL11A1和Wnt4的表达水平上调，而COL4A4的表达被下调了。从rBMSCs分离出的外泌体可改善急性软组织损伤，并可作为急性软组织损伤的新治疗策略。