W34 is a prodrug of FL118, and it can be converted to FL118 via a hydrolysis reaction. In this report, a highly sensitive LC–MS/MS method using a C₁₈ column was validated and used for the simultaneous determination of W34 and FL118 in rat blood. A stepwise gradient elution with 0.1% formic acid in water and acetonitrile was employed. The assays were linear over a concentration range of 0.50–50.0 ng/ml for both W34 and FL118. The accuracy of the validation method ranged from 89.74 to 98.94% for W34 and from 88.61 to 94.60% for FL118. The precision was within 7.15% for W34 and 9.63% for FL118. Extraction recoveries of W34 were 94.56–100.49 and 87.67–106.32% for FL118. No significant matrix effects for both W34 and FL118 were observed in blood. The assay has been successfully applied to biological samples obtained from a stability and pharmacokinetic study of W34 and FL118.

中文翻译：

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通过LC-MS / MS同时测定大鼠血液中FL118和W34：在药代动力学研究中的应用。

W34是FL118的前药，并且可以通过水解反应转化为FL118。在本报告中，使用C ₁₈的高灵敏度LC-MS / MS方法色谱柱经过验证，可用于同时测定大鼠血液中的W34和FL118。使用在水中和乙腈中的0.1％甲酸的逐步梯度洗脱。W34和FL118的浓度在0.50-50.0 ng / ml范围内呈线性关系。验证方法的准确性，对于W34，范围为89.74至98.94％，对于FL118，范围为88.61至94.60％。W34的精度在7.15％之内，FL118的精度在9.63％之内。FL118的W34提取回收率为94.56–100.49和87.67–106.32％。在血液中未观察到W34和FL118的显着基质效应。该测定法已成功应用于从W34和FL118的稳定性和药代动力学研究获得的生物样品。