The benzotriazole UV stabilizer (BUVS) 2-(5-chloro-benzotriazol-2-yl)-4,6-di-(tert-butyl)phenol (UV-327) is used in various plastic products to protect them against harmful UV radiation. Meanwhile, there are concerns about potential adverse health effects on humans, as residues of UV-327 and other BUVSs have already been detected in various environmental matrices. However, information on the metabolism of UV-327 is not yet available. Therefore, in vitro experiments with human liver microsomes (HLMs) were performed in order to identify phase I metabolites to be used as specific biomarkers of exposure in biomonitoring studies. The samples were analyzed by HPLC coupled with mass spectrometry (HPLC/MS). Potential metabolites, which were formed by hydroxylation and further oxidation to carboxylic acid, were tentatively identified. Special metabolite structures were suspected and custom-synthesized as reference substances for verification. In total, seven phase I metabolites, which may be suitable biomarkers for the assessment of exposure to UV-327, have been identified and quantified. The results of the present study provide initial insights into the metabolic pathway of UV-327, which is essential for further research on its human metabolism.

苯并三唑紫外线稳定剂（BUVS）2-（5-氯-苯并三唑-2-基）-4,6-二-（叔丁基）苯酚（UV-327）用于各种塑料产品中，以保护它们免受有害的紫外线侵害辐射。同时，由于已经在各种环境基质中检测到UV-327和其他BUVS的残留，因此对人体潜在的不利健康影响存在担忧。但是，尚无关于UV-327代谢的信息。因此，体外进行了人类肝微粒体（HLM）实验，以鉴定在生物监测研究中用作暴露的特定生物标志物的I期代谢物。通过HPLC结合质谱法（HPLC / MS）分析样品。初步鉴定了通过羟基化和进一步氧化为羧酸形成的潜在代谢物。怀疑特殊的代谢产物结构并定制合成为参考物质以进行验证。总共已经鉴定和定量了七种I期代谢物，它们可能是评估暴露于UV-327的合适生物标志物。本研究的结果提供了对UV-327代谢途径的初步见解，这对进一步研究其人类代谢至关重要。