In this study, fragments of polyethylene plastic bags were treated with simulated gastric juice of fish for 16 hr. Following solid-phase extraction, methanol eluents caused acute toxicity to embryos and larvae of Japanese medaka. Chromatographic fractions (polar to more non-polar with numbers increasing) of the extract were evaluated for toxicity and estrogenic activity using medaka and an estrogen receptor (ER) cell-line. Fractions 6 and 9 had the highest estrogenic effects with relative hydrophobic chemicals. The vtg expression in fraction 6 was 22-fold higher than control, and the ER cellular response in fraction 9 was 8.5-fold higher than controls. Following non-target screening (NTS), several novel phthalates and phenols were identified in the above two fractions. Fractions 1 and 2 appeared to be primarily responsible for the acute toxicity observed with the whole extract. The hatching rate decreased to 36% in fraction 2, and the hatching of medaka larvae from exposed embryos was even not observed following exposure to fraction 1. NTS of these fractions indicated 635 and 808 entities, respectively, most without toxicity information. These results indicate plastic leachates from gastric juices of fish are complex mixtures of many compounds that can have acute reproductive and sublethal endocrine impacts in fish.

在这项研究中，将聚乙烯塑料袋的碎片用模拟鱼胃液处理16小时。固相萃取后，甲醇洗脱液对日本med的胚胎和幼虫产生急性毒性。使用medaka和雌激素受体（ER）细胞系评估提取物的色谱级分（极性至更多非极性，且数量增加）的毒性和雌激素活性。馏分6和9在相对疏水的化学物质中具有最高的雌激素作用。该VTG在级分6中的表达比对照高22倍，在级分9中的ER细胞应答比对照高8.5倍。经过非目标筛选（NTS）后，在上述两个馏分中鉴定出了几种新型邻苯二甲酸酯和酚。馏分1和2似乎是造成整个提取物急性毒性的主要原因。在级分2中，孵化率降低到36％，暴露于级分1后甚至没有观察到从暴露的胚中孵出的med幼虫。这些级分的NTS分别指示635和808个实体，大多数没有毒性信息。这些结果表明，鱼胃液中的塑料渗滤液是许多化合物的复杂混合物，会对鱼类产生急性生殖和亚致死性内分泌影响。