Novel hyper-resistant bacteria were isolated from the Crven Dol mine (Allchar, North Macedonia), arsenic-rich extreme environment. Bacteria were recovered from a secondary mineral mixture, an alteration of hydrothermal realgar rich in arsenates (pharmacolite, hornesite, and talmessite). The sample was recovered from the dark part of the mine at 28 m depth. Three bacterial strains and a bacterial consortium were isolated for their capacity to survive exposure to 32 g/L (209 mM) of arsenite, and 176 g/L (564 mM) of arsenate. The 16S rRNA gene analysis identified bacterial isolates as Stenotrophomonas sp. and two Microbacterium spp. This analysis also revealed that bacterial consortium comprise two Bacteriodetes exhibiting similarity to Olivibacter ginsengisoli and to uncultured bacterium, and one γ-proteobacteria with similarity to Luteimonas sp. Among all isolates Stenotrophomonas sp. exhibited the highest tolerance to As compound as well as the capacity to accumulate As inside the cells. Analysis of genes involved in As-resistance showed that recovered isolates possess the genes encoding the ArsB, Acr3(1) and Acr3(2) proteins, indicating that at least a part of their resistance could be ascribed to As-efflux systems described in isolates obtained from human-polluted environments.

从Crven Dol矿（北马其顿Allchar），富含砷的极端环境中分离出了新型的高抗细菌。从次要矿物混合物中回收细菌，这是富含砷酸盐（药石，角质石和滑石）的热液雄黄的替代物。从矿山的黑暗部分以28 m的深度回收样品。分离了三种细菌菌株和细菌聚生体，以使其能够耐受暴露于32 g / L（209 mM）的砷和176 g / L（564 mM）的砷酸盐的影响。16S rRNA基因分析鉴定出细菌分离株为Stenotrophomonas sp。和两个微杆菌属。该分析还揭示了细菌聚生体包含两个与人参寡糖相似的细菌以及未培养细菌和一种与Luteimonas sp。类似的γ-变形杆菌。在所有分离株中，嗜单胞菌属。表现出对As化合物的最高耐受性以及在细胞内积累As的能力。对与抗性有关的基因的分析表明，回收的分离株具有编码ArsB，Acr3（1）和Acr3（2）蛋白的基因，表明它们的至少部分抗性可能归因于分离株中所述的As-外排系统。从人类污染的环境中获得。