In this study, we characterized the transcriptome and chloroplast genome of Glycyrrhiza inflata and performed comparative analyses with G. uralensis and G. glabra. 60,541unigenes were obtained from the transcriptome of G. inflata. The results of function annotation revealed a similar distribution of functional categories among three licorice species. By comparing chloroplast genomes of licorice species, it was demonstrated that the structure and the length of genome as well as gene content and gene order were highly similar. The phylogenetic tree, constructed with the mixed data of transcriptome and chloroplast genome, elucidated that G. inflata and G. glabra had a closer relationship than G. uralensis. Six regions were suggested as potential markers for the identification of three licorice species. In each licorice species, two unigenes were homologous to reference flavonol synthase. For G. inflata, 48 and 21 RNA editing sites were detected by PREP-Cp program and RNA-Seq data mapping, respectively.

在这项研究中，我们表征了膨胀甘草的转录组和叶绿体基因组，并与G. uralensis和G. glabra进行了比较分析。从G. inflata的转录组中获得了 60,541 个unigenes 。功能注释的结果揭示了三种甘草物种之间功能类别的相似分布。通过比较甘草种类的叶绿体基因组，证明其基因组的结构和长度以及基因含量和基因顺序高度相似。用转录组和叶绿体基因组的混合数据构建的系统发育树阐明了G. inflata和G. glabra与G. uralensis有更密切的关系。六个区域被建议作为鉴定三种甘草品种的潜在标记。在每个甘草物种中，两个 unigenes 与参考黄酮醇合酶同源。对于G. inflata，通过 PREP-Cp 程序和 RNA-Seq 数据映射分别检测到 48 个和 21 个 RNA 编辑位点。