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Metabolic acclimation of anammox consortia to decreased temperature
Environment International ( IF 10.3 ) Pub Date : 2020-07-08 , DOI: 10.1016/j.envint.2020.105915 Tangran Huo 1 , Yunpeng Zhao 1 , Xi Tang 1 , Huazhang Zhao 2 , Shouqing Ni 3 , Qiang Gao 2 , Sitong Liu 4
Environment International ( IF 10.3 ) Pub Date : 2020-07-08 , DOI: 10.1016/j.envint.2020.105915 Tangran Huo 1 , Yunpeng Zhao 1 , Xi Tang 1 , Huazhang Zhao 2 , Shouqing Ni 3 , Qiang Gao 2 , Sitong Liu 4
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Widespread application of anammox process has been primarily limited to the high sensitivity of anammox consortia to fluctuations of temperature. However, the metabolic acclimation of anammox consortia to decreased temperature remains unclear, which is the core of developing potential strategies for improving their low-temperature resistance. Here, we operated anammox reactors at 25 °C and 35 °C to explore the acclimation mechanism of anammox consortia in terms of metabolic responses and cross-feedings. Accordingly, we found that the adaptation of anammox consortia to ambient temperature (25 °C) was significantly linked to energy conservation strategy, resulting in decreased extracellular polymeric substance secretion, accumulation of ATP and amino acids. The expression patterns of cold shock proteins and core enzymes caused the apparent metabolic advantage of Candidatus for acclimation to ambient temperature compared to other anammox species. Importantly, strengthened cross-feedings of amino acids, nitrite and glycine betaine benefited adaptation of anammox consortia to ambient temperature. Our work not only uncovers the temperature-adaptive mechanisms of anammox consortia, but also emphasizes the important role of metabolic cross-feeding in the temperature adaptation of microbial community.
中文翻译：
厌氧氨氧化菌群对降低温度的代谢适应
厌氧氨氧化工艺的广泛应用主要限于厌氧氨氧化菌群对温度波动的高敏感性。然而，厌氧氨氧化菌群对低温的代谢适应仍不清楚，这是开发提高其耐低温性的潜在策略的核心。在这里，我们在 25 °C 和 35 °C 下运行厌氧氨氧化反应器，以探索厌氧氨氧化菌群在代谢反应和交叉喂养方面的驯化机制。因此，我们发现厌氧氨氧化菌群对环境温度（25℃）的适应与节能策略显着相关，导致细胞外聚合物分泌减少、ATP和氨基酸积累减少。与其他厌氧氨氧化物种相比，冷休克蛋白和核心酶的表达模式导致 Candidatus 在适应环境温度方面具有明显的代谢优势。重要的是，加强氨基酸、亚硝酸盐和甘氨酸甜菜碱的交叉供给有利于厌氧氨氧化菌群对环境温度的适应。我们的工作不仅揭示了厌氧氨氧化菌群的温度适应机制，而且强调了代谢交叉摄食在微生物群落温度适应中的重要作用。
更新日期：2020-07-08
中文翻译：
厌氧氨氧化菌群对降低温度的代谢适应
厌氧氨氧化工艺的广泛应用主要限于厌氧氨氧化菌群对温度波动的高敏感性。然而，厌氧氨氧化菌群对低温的代谢适应仍不清楚，这是开发提高其耐低温性的潜在策略的核心。在这里，我们在 25 °C 和 35 °C 下运行厌氧氨氧化反应器，以探索厌氧氨氧化菌群在代谢反应和交叉喂养方面的驯化机制。因此，我们发现厌氧氨氧化菌群对环境温度（25℃）的适应与节能策略显着相关，导致细胞外聚合物分泌减少、ATP和氨基酸积累减少。与其他厌氧氨氧化物种相比，冷休克蛋白和核心酶的表达模式导致 Candidatus 在适应环境温度方面具有明显的代谢优势。重要的是，加强氨基酸、亚硝酸盐和甘氨酸甜菜碱的交叉供给有利于厌氧氨氧化菌群对环境温度的适应。我们的工作不仅揭示了厌氧氨氧化菌群的温度适应机制，而且强调了代谢交叉摄食在微生物群落温度适应中的重要作用。
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