Chemiluminescence and fluorescence have revolutionized the study of chemical and biological processes. However, unlike fluorescence, spatiotemporal control of chemiluminescence is challenging, and the technically simple solution of converting chemical energy into light energy only at specific sites defined by a clean, easy-to-deliver focused light stimulus is not yet a viable option. Here, we demonstrate the spatial and temporal control of a chemiluminescent reaction by photoinduced oxidative hotspots on a silicon surface. The excitation light is not required to pass through the liquid sample; hence, the light stimulus can excite a luminescent dye under effectively dark conditions. This provides a strategy for removing light-induced sample damages, photobleaching, and autofluorescence.

化学发光和荧光已经彻底改变了化学和生物过程的研究。但是，与荧光不同，化学发光的时空控制具有挑战性，仅在清洁，易于传递的聚焦光刺激所定义的特定位置将化学能转化为光能的技术上简单的解决方案仍不是可行的选择。在这里，我们演示了由硅表面上的光诱导氧化热点对化学发光反应的空间和时间控制。激发光不需要穿过液体样品；因此，光刺激可以在有效的黑暗条件下激发发光染料。这提供了消除光引起的样品损伤，光漂白和自发荧光的策略。