Cytochrome P450 (CYP) enzymes play important roles in the detoxification and bioactivation of environmental contaminants and are involved in the responses to pollution in fish. In this study, we cloned four new CYP1 genes, CYP1A, CYP1B, CYP1C1, and CYP1C2, from black rockfish (Sebastes schlegelii), a dominant and economically important fish species in Korea. This species is at a significant risk of exposure to petrohydrocarbons, such as benzo[a]pyrene(B[a]P), due to frequent oil spills along the Korean coast. Quantitative PCR analysis of CYP1 gene transcription in 12 organs of the fish revealed tissue-specific expression patterns. CYP1A was significantly expressed in the liver, heart, kidneys, and muscle, and CYP1B was significantly expressed in the gills, muscle, and heart. CYP1C1 and CYP1C2 showed similar tissue expression patterns, with the highest levels in the muscle. Furthermore, exposure to an aryl hydrocarbon receptor (AHR) agonist, B[a]P, at 2, 20, and 200 mg/kg body weight showed significant dysregulation of the CYP1A, CYP1B, CYP1C1, and CYP1C2 expression levels in the gills, liver, kidneys, and spleen. The mRNA expression levels of CYP1A and CYP1B were upregulated by 450- and 17-fold, respectively, in the spleen. Compared with their levels in the control, CYP1C1 increased by 45-fold, while CYP1C2 remained unchanged in the gills, indicating differential effects of the polycyclic aromatic hydrocarbon on CYP1 expression in different fish organs. The results suggested that expression profiles of inducible CYP1 enzymes in S. schlegelii might be used as indicators for assessing aquatic contamination by AHR agonists. Determination of the basal and induced expression levels, as well as substrate specificity, of the four CYP1 enzymes may contribute to a better understanding of their roles in the metabolization of toxicants or drugs.

细胞色素P450（CYP）酶在环境污染物的解毒和生物活化中起重要作用，并参与对鱼类污染的反应。在这项研究中，我们从黑rock鱼（Sebastes schlegelii）中克隆了四个新的CYP1基因CYP1A，CYP1B，CYP1C1和CYP1C2。由于朝鲜沿岸经常发生溢油事故，该物种极有可能接触石油烃，例如苯并[a] py（B [a] P）。鱼类12个器官中CYP1基因转录的定量PCR分析揭示了组织特异性表达模式。CYP1ACYP1B在肝，心脏，肾脏和肌肉中显着表达，而CYP1B在the，肌肉和心脏中显着表达。CYP1C1和CYP1C2显示相似的组织表达模式，在肌肉中含量最高。此外，暴露于2、20和200 mg / kg体重的芳烃受体（AHR）激动剂B [a] P显示g中的CYP1A，CYP1B，CYP1C1和CYP1C2表达水平显着失调，肝脏，肾脏和脾脏。CYP1A和CYP1B的mRNA表达水平在脾脏中分别被上调了450倍和17倍。与对照组相比，CYP1C1增加了45倍，而中的CYP1C2保持不变，表明多环芳香烃对不同鱼类器官中CYP1表达的不同影响。结果表明，在诱导CYP1酶的表达谱S.许氏可能被用来作为指标通过AHR激动剂评估水生污染。确定四种CYP1酶的基础表达水平和诱导表达水平以及底物特异性可能有助于更好地了解它们在有毒物质或药物代谢中的作用。