Herein, Fe3O4 NP@ZIF-8/MoS2 QD-based electrochemiluminescence (ECL) biosensor with nanosurface energy transfer strategy was successfully developed for point-of-care determination of ATP. With the porous structure and poor electron transfer ability, Fe3O4 NP@ZIF-8 complex was first used as an excellent catalyst in ECL. The complex catalyzed the coreactant for more free radicals and hindered the quenching effect of Fe3O4 nanoparticles (NPs) on quantum dots (QDs). In ECL-nanosurface energy transfer (NSET) system, through the specific binding of complementary DNA linked to MoS2 QDs (QDs-cDNA) and aptamer linked to Au NPs, interaction between the point dipole of MoS2 QDs and the collective dipoles of Au NPs quenched ECL signal. When ATP was captured by aptamer, the ECL-NSET system was taken apart, which resulted in the recovery of ECL signal. Moreover, changes of the ECL imaging can be captured by a smartphone, which enabled point-of-care determination of ATP from 0.05 nmol L-1 to 200 nmol L-1 with LOD of 0.015 nmol L-1. With superior specificity and stability, the sensing system showed significant potential about the application of catalysts coated with ZIF and NSET in point-of-care ECL determination.

中文翻译：

---

基于 Fe3O4 NP@ZIF-8/MoS2 QD 的电化学发光与纳米表面能量转移策略用于 ATP 的即时测定

在此，成功开发了具有纳米表面能量转移策略的基于 Fe3O4 NP@ZIF-8/MoS2 QD 的电化学发光 (ECL) 生物传感器，用于 ATP 的即时测定。Fe3O4 NP@ZIF-8 复合物具有多孔结构和较差的电子转移能力，首次被用作 ECL 中的优良催化剂。该配合物催化共反应物产生更多自由基，并阻碍 Fe3O4 纳米粒子 (NPs) 对量子点 (QD) 的淬灭作用。在 ECL-纳米表面能量转移 (NSET) 系统中，通过与 MoS2 QDs (QDs-cDNA) 相连的互补 DNA 和与 Au NPs 相连的适体的特异性结合，MoS2 QDs 的点偶极子和 Au NPs 的集体偶极子之间的相互作用被淬灭ECL 信号。当 ATP 被适配体捕获时，ECL-NSET 系统被拆开，导致 ECL 信号的恢复。而且，ECL 成像的变化可以通过智能手机捕捉到，这使得 ATP 的即时测定范围从 0.05 nmol L-1 到 200 nmol L-1，LOD 为 0.015 nmol L-1。凭借卓越的特异性和稳定性，传感系统显示出将涂有 ZIF 和 NSET 的催化剂应用于床旁 ECL 测定的巨大潜力。