The efficient extraction and fixation of a tissue allows in preserving the cytoarchitecture, chemical composition and tissue organization, which is key in physiological and histopathological studies. The main goal of this study was to establish a microsurgery technique to obtain ocular tissue and provide an optimized immersion fixation protocol based on the 10 % formalin-intraocular injection on Olive ridley sea turtle hatchlings (Lepidochelys olivacea). To evaluate this optimized technique, a histological comparison between traditional immersion and intraocular/immersion protocols was done. The eyeball were processed into five protocols: Frozen eyes (Group 1), frozen eyes immersed in 10 % formalin (Group 2), fresh eyes immersed in 10 % formalin (Group 3), fresh eyes intraocularly injected with 0.1 M phosphate buffer solution (PBS) and then immersed in 10 % formalin (Group 4), and fresh eyes fixed by 10 % formalin-intraocular followed by 10 % formalin-immersion (Group 5). In comparison with all groups evaluated, the intraocular/immersion fixation protocol lead the conservation of eyeball shape, cell integrity and maintenance of the organization of the retina layers of sea turtle hatchlings. If this method will be the key in studying sea turtle, we suppose that this procedure, with minimal adjustments, could be useful in animals with similar eye anatomy.

有效提取和固定组织可以保留细胞结构，化学成分和组织结构，这在生理学和组织病理学研究中至关重要。这项研究的主要目的是建立一种显微手术技术，以获取眼部组织，并基于10％福尔马林-眼内注射法对Olive ridley海龟幼体（Lepidochelys olivacea）提供优化的浸入固定方案）。为了评估这种优化的技术，在传统浸入法和眼内/浸入法之间进行了组织学比较。将眼球分为五种方案：冷冻眼（第1组），浸入10％福尔马林的冷冻眼（第2组），浸入10％福尔马林的新鲜眼（第3组），眼内注射0.1 M磷酸盐缓冲液的新鲜眼（ PBS），然后浸入10％福尔马林中（第4组），然后用10％福尔马林眼内固定的新鲜眼睛，然后浸入10％福尔马林中（组5）。与评估的所有组相比，眼内/浸入固定方案可保护眼球形状，保持细胞完整性并维持海龟幼体视网膜层的组织。如果这种方法将成为研究海龟的关键，那么我们假设该程序