Abstract

A liquid chromatography−mass spectrometry method was developed for the simultaneous quantitation of (2R,3R)-Dihydromyricetin and Myricetin in rat plasma samples to facilitate analysis of samples collected after administration of SenDeng-4 medicine. The plasma was subjected to protein precipitation with HCl (containing 10% ascorbic acid) and analyzed using electrospray ionization mass spectrometry in selected ion monitoring mode with Paeoniflorin as an internal standard. Good linearity for the two compounds was observed. The intra- and inter-day precision of analysis was <10% for each compound. The present method was suitable for determining (2R,3R)-Dihydromyricetin and Myricetin in rat plasma after intravenous administration of SenDeng-4 to rats with sufficient sensitivity.

中文翻译：

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液相色谱-质谱法同时测定大鼠血浆中蒙古药Sendeng-4的（2R，3R）-二氢杨梅素和杨梅素

摘要

建立了液相色谱-质谱联用法同时定量大鼠血浆样品中的（2R，3R）-二氢杨梅素和杨梅素的方法，以利于分析使用SenDeng-4药物后收集的样品。用HCl（含10％的抗坏血酸）对血浆进行蛋白质沉淀，并使用电喷雾电离质谱法在选定的离子监测模式下以Pa药苷作为内标进行分析。观察到两种化合物的线性良好。每种化合物的日内和日间分析精度均<10％。本方法适用于以足够的敏感性向大鼠静脉内注射SenDeng-4后测定大鼠血浆中的（2R，3R）-二氢杨梅素和杨梅素。