To investigate the influence of jasmonic acid (JA) on morpho-physiological and biochemical characteristics of strawberry cv. Queen Elisa, under in vitro PEG-induced water stress, a factorial experiment based on completely randomized design (CRD) was conducted. PEG-induced water stress was imposed using polyethylene glycol (PEG6000) at three levels (0, 5 and 7% w/v), under three levels of JA application (0, 0.01 and 0.05 mM). The results indicated that PEG-induced water stress negatively impacted all studied morphological traits except for shoot fresh and dry weight. Furthermore, PEG-induced water stress had a decreasing effect on physiological traits including relative water content (RWC), photosynthesis pigments, membrane-stability index (MSI), and total proteins while it increased the activity of peroxidase (POD) and superoxide dismutase (SOD) enzyme and the content of malondialdehyde (MDA), hydrogen peroxide (H₂O₂), and proline. JA application showed an interaction effect with PEG-induced water stress on all studied traits except for SOD, POD, and total carbohydrate. Besides, based on the heat-map and principal component analysis results, three distinct groups were observed for physio-biochemical traits. Accordingly, the most effective treatment was the application of 0.05 mM JA under non-stressed condition. Altogether, the strawberry plants treated with higher JA concentrations could effectively cope with PEG-induced water stress adverse effects. Meanwhile, JA application improved strawberry growth attributes under both stress and non-stress conditions. In conclusion, the application of JA can be suggested as an effective way to alleviate deleterious impacts dehydration stress on strawberry under in vitro conditions.

研究茉莉酸（JA）对草莓简历形态生理生化特性的影响。Queen Elisa，在体外PEG诱导的水分胁迫下，进行了基于完全随机设计（CRD）的析因实验。在三个水平的JA施用（0、0.01和0.05 mM）下，使用三个水平（0、5和7％w / v）的聚乙二醇（PEG6000）施加PEG诱导的水分胁迫。结果表明，PEG诱导的水分胁迫对所有研究的形态性状均具有负面影响，除了茎干和鲜重。此外，PEG诱导的水分胁迫对生理特性的影响降低，这些生理特性包括相对含水量（RWC），光合作用色素，膜稳定性指数（MSI），₂ O ₂）和脯氨酸。JA的应用显示了与PEG引起的水分胁迫对除SOD，POD和总碳水化合物外的所有研究性状的相互作用。此外，根据热图和主成分分析结果，观察到三个不同的组的生理生化特征。因此，最有效的治疗方法是在非压力条件下施用0.05 mM JA。总之，用较高的JA浓度处理的草莓植株可以有效应对PEG引起的水分胁迫的不利影响。同时，JA施用改善了在胁迫和非胁迫条件下的草莓生长特性。总之，JA的应用可以被认为是减轻草莓在体外条件下脱水胁迫的有害影响的有效途径。