An efficient in vitro plant regeneration method via somatic embryogenesis has been established in Ormosia henryi Prain, which is an important precious tree in the timber industry. Five types of explants, six types of basic media and different plant growth regulators (PGRs) were used for embryogenic callus (EC) initiation, EC proliferation, somatic embryo (SE) induction and SE germination. The EC and SE induction rates from mature zygotic embryos (MZE) were higher than those from stem segments, cotyledons, leaves and hypocotyls. MZE were used as explants, and the EC induction rate was greater than 30% on B5 medium supplemented with 1.0 mg/l 6-benzylaminopurine (BA) and 0.5 mg/l naphthaleneacetic acid (NAA) or 0.2 mg/l BA and 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The highest induction rate (22.83%) for SE was obtained with B5 medium supplemented with 0.5 mg/l kinetin (KT) and 1.0 mg/l 2,4-D. The EC proliferation rate and SE number in the suspension culture were increased compared to those in the solid culture. Fifty-four percent of SE developed to the cotyledonary stage on B5 medium supplemented with 0.5 mg/l BA and 0.2 mg/l NAA. The number of cotyledon embryos was the highest on medium supplemented with 0.5 mg/l thidiazuron (TDZ) and 0.2 mg/l NAA. Histological analysis confirmed the bipolar organization of the somatic embryos, which were comprised of shoot and root meristems, and showed that SE was characterized by globular, heart-shaped, torpedo-shaped and cotyledon stages in O. henryi, the developmental process of which was similar to that of zygotic embryos.

在Ormosia henryi中建立了通过体细胞胚发生的有效的体外植物再生方法培林，是木材工业中重要的珍贵树种。使用五种外植体，六种基本培养基和不同的植物生长调节剂（PGR）进行胚发生愈伤组织（EC）的引发，EC增殖，体细胞胚（SE）诱导和SE发芽。成熟合子胚（MZE）的EC和SE诱导率高于茎节，子叶，叶片和下胚轴。MZE被用作外植体，并且在补充了1.0 mg / l的6-苄氨基嘌呤（BA）和0.5 mg / l的萘乙酸（NAA）或0.2 mg / l的BA和2.0 mg的B5培养基上，EC诱导率大于30％。 / l 2，4-二氯苯氧基乙酸（2，4-D）。B5培养基添加0.5 mg / l激动素（KT）和1.0 mg / l 2,4-D可获得SE的最高诱导率（22.83％）。与固体培养相比，悬浮培养的EC增殖率和SE数增加。在补充有0.5 mg / l BA和0.2 mg / l NAA的B5培养基上，SE的54％发育到子叶期。在补充有0.5 mg / l噻唑隆（TDZ）和0.2 mg / l NAA的培养基上，子叶胚的数量最高。组织学分析证实了体细胞胚的双极组织，其由芽和根分生组织组成，并表明SE的特征是球形，心形，鱼雷形和子叶期。在补充有0.5 mg / l噻唑隆（TDZ）和0.2 mg / l NAA的培养基上，子叶胚的数量最高。组织学分析证实了体细胞胚的双极组织，其由芽和根分生组织组成，并表明SE的特征是球形，心形，鱼雷形和子叶期。在补充有0.5 mg / l噻唑隆（TDZ）和0.2 mg / l NAA的培养基上，子叶胚的数量最高。组织学分析证实了体细胞胚的双极组织，其由芽和根分生组织组成，并表明SE的特征是球形，心形，鱼雷形和子叶期。O. henryi，其发育过程与合子胚相似。