Acidic pH of lysosomes is closely related to autophagy; thus, well known of the precise lysosomes, pH changes will give more information on the autophagy process and status. So far, however, only pH changes in a relatively broad range could be indicated, the exact lysosomes pH detection has never arrived. In our study, we established an endo/lysosome pH indicator based on the self-decomposable SiO₂ nanoparticle system with specific synthesis parameters. The central concentrated methylene blue (MB) in the central-hollow structural nanoparticles presented sensitive release as a function of pH values from pH 4.0–4.8, which is exactly the pH range of lysosomes. The linear correlation of the optical density (OD) values and the pH values has been built up, which has been used for the detection of lysosomes pH in 6 different cell lines. Moreover, by this system, we succeeded in precisely detecting the pH average changes of lysosomes before and after black mesoporous silicon (BPSi) NP endocytosis, clarifying the mechanism of the autophagy termination after BPSi endocytosis. So, the self-decomposable nanoparticle-based luminal pH indicator may provide a new methodology and strategy to know better of the lysosome pH, then indicate more details on the autophagy process or other important signaling about metabolisms.

溶酶体的酸性pH值与自噬密切相关。因此，众所周知的精确溶酶体，pH值的变化将提供有关自噬过程和状态的更多信息。但是，到目前为止，只能指示相对较大范围内的pH值变化，而准确的溶酶体pH检测从未到过。在我们的研究中，我们基于可自分解的SiO ₂建立了内在/溶酶体pH指示剂具有特定合成参数的纳米粒子系统。中央空心结构纳米颗粒中的中央浓缩亚甲基蓝（MB）随pH 4.0-4.8（恰好是溶酶体的pH范围）的pH值而呈现敏感释放。建立了光密度（OD）值与pH值的线性相关性，该线性相关性已用于检测6种不同细胞系中的溶酶体pH。此外，通过该系统，我们成功地精确检测了黑色介孔硅（BPSi）NP内吞之前和之后的溶酶体的pH平均变化，阐明了BPSi内吞后自噬终止的机制。因此，可自分解的基于纳米颗粒的腔内pH指示剂可提供一种新的方法和策略，以更好地了解溶酶体的pH，