NACs are one of the major transcription factor families in plants which play an important role in plant growth and development, as well as in adverse stress responses. In this study, we cloned a salt-inducible NAC transcription factor gene (NAC13) from a poplar variety 84K, followed by transforming it into both Nicotiana tabacum and Arabidopsis thaliana. Stable expression analysis of 35S::NAC13-GFP fusion protein in Arabidopsis indicated that NAC13 protein was localized to the nucleus. We also obtained five transgenic tobacco lines. Evidence from morphological and physiological characterization and salt treatment analyses indicated that in the transgenic tobacco the salt tolerance was enhanced, suggesting that NAC13 gene may function as a positive regulator in tobacco responses to salt stress.

NAC是植物中主要的转录因子家族之一，其在植物生长和发育以及逆境胁迫应答中起重要作用。在这项研究中，我们从杨树品种84K中克隆了盐诱导的NAC转录因子基因（NAC13），然后将其转化为烟草和拟南芥。35S :: NAC13-GFP融合蛋白在拟南芥中的稳定表达分析表明，NAC13蛋白位于细胞核中。我们还获得了五个转基因烟草品系。形态和生理特征以及盐处理分析的证据表明，在转基因烟草中，盐分耐受性增强，表明NAC13 该基因可能在烟草对盐胁迫的反应中起正调控作用。