The shikimate pathway is indispensable for the biosynthesis of natural products with aromatic moieties. These products have wide current and potential applications in food, cosmetics and medicine, and consequently have great commercial value. However, compounds extracted from various plants or synthesized from petrochemicals no longer satisfy the requirements of contemporary industries. As a result, an increasing number of studies has focused on this pathway to enable the biotechnological manufacture of natural products, especially in E. coli. Furthermore, the development of synthetic biology, systems metabolic engineering and high flux screening techniques has also contributed to improving the biosynthesis of high-value compounds based on the shikimate pathway. Here, we review approaches based on a combination of traditional and new metabolic engineering strategies to increase the metabolic flux of the shikimate pathway. In addition, applications of this optimized pathway to produce aromatic amino acids and a range of natural products is also elaborated. Finally, this review sums up the opportunities and challenges facing this field.

iki酸酯途径对于具有芳族部分的天然产物的生物合成是必不可少的。这些产品在食品，化妆品和药品中具有广泛的当前和潜在应用，因此具有巨大的商业价值。然而，从各种植物中提取或从石化产品合成的化合物不再满足当代工业的要求。结果，越来越多的研究集中在这种途径上，以实现生物技术生产天然产物，特别是在大肠杆菌中。此外，合成生物学，系统代谢工程学和高通量筛选技术的发展也有助于改善基于the草酸酯途径的高价值化合物的生物合成。在这里，我们回顾了基于传统和新陈代谢工程策略相结合的方法，以增加the草酸酯途径的代谢通量。此外，还详细说明了该优化途径在生产芳香族氨基酸和一系列天然产物中的应用。最后，本综述总结了该领域面临的机遇和挑战。