Corn gluten meal (CGM) was hydrolyzed by Alcalase 2.4L and Protex 7L to obtain low-molecular-weight peptides. The antioxidant and angiotensin-I-converting enzyme (ACE) inhibitory activities of the peptides were determined by 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free radical scavenging activity, the JHH7 cell oxidative stress model, and the ACE inhibition assay, respectively. Corn peptides (CPs) had strong antioxidant capacity to scavenge ABTS radicals (1.44 ± 0.11 mmol/g), and significantly reduced reactive oxygen species and malondialdehyde levels in oxidized JHH7 cells, increasing superoxide dismutase, catalase, and glutathione peroxidase activities by 26.20%, 147.52%, and 43.20%, respectively, at the concentration of 800 μg/mL. CPs had high ACE inhibitory activity, with an IC₅₀ value of 0.27 ± 0.05 mg/mL. Next, the CPs were separated by reverse-phase high-performance liquid chromatography. Twelve fractions were collected, and their ABTS free radical scavenging and ACE inhibitory activities were determined. Fractions 1, 2, 3, 4, and 9 showed higher ABTS free radical scavenging and ACE inhibitory activities. Twelve peptides were identified from active fractions by liquid chromatography–tandem mass spectrometry (LC–MS/MS) as Leu-Ser-Pro-Tyr, Leu-Asn-Ser-Pro-Ala-Tyr, Tyr-Gly-Pro-Gln, Pro-Pro-Tyr, Ala-Tyr-Pro-Glu, Thr-Tyr-Ser-Gly-Pro-Lys, Ala-Tyr-Pro-Gly-Pro-Gln, Leu-Ala-Tyr-Pro-Gln, Ala-Tyr-Leu-Gln-Gln-Gln, Ser-Ala-Pro, Asn-Ala-Pro, and Val-Asn-Ala-Pro. These peptides exhibited good ABTS free radical scavenging and ACE inhibitory activities. Therefore, CPs may have potential applications in the food industry as functional material additives.

中文翻译：

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玉米蛋白粉肽的体外抗氧化和血管紧张素转化酶抑制特性

用Alcalase 2.4L和Protex 7L水解玉米蛋白粉（CGM），以获得低分子量肽。通过2,2'-Azinobis-（3-乙基苯并噻唑啉-6-磺酸盐）（ABTS）自由基清除活性，JHH7细胞氧化应激模型确定肽的抗氧化剂和血管紧张素-I转换酶（ACE）抑制活性，和ACE抑制分析。玉米肽（CP）具有强大的抗氧化能力，可清除ABTS自由基（1.44±0.11 mmol / g），并显着降低氧化的JHH7细胞中的活性氧和丙二醛含量，使超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶活性增加26.20％，在800μg/ mL的浓度下分别为147.52％和43.20％。CP具有较高的ACE抑制活性，IC ₅₀值为0.27±0.05 mg / mL。接下来，通过反相高效液相色谱法分离CP。收集十二个馏分，并测定它们的ABTS自由基清除和ACE抑制活性。组分1、2、3、4和9显示出更高的ABTS自由基清除能力和ACE抑制活性。通过液相色谱-串联质谱（LC-MS / MS）从活性级分中鉴定出十二种肽，分别为Leu-Ser-Pro-Tyr，Leu-Asn-Ser-Pro-Ala-Tyr，Tyr-Gly-Pro-Gln， Pro-Pro-Tyr，Ala-Tyr-Pro-Glu，Thr-Tyr-Ser-Gly-Pro-Lys，Ala-Tyr-Pro-Gly-Pro-Gln，Leu-Ala-Tyr-Pro-Gln，Ala- Tyr-Leu-Gln-Gln-Gln，Ser-Ala-Pro，Asn-Ala-Pro和Val-Asn-Ala-Pro。这些肽表现出良好的ABTS自由基清除和ACE抑制活性。因此，