The generation of growth curves is a common and established method for determining the properties of bacterial strains. Such properties can be growth rates in enrichment broths or resistance to antibiotics. Enrichment methods are the gold standard for enumerating bacteria and determining growth properties. Newer methods can use agents that are metabolized during bacterial growth, which leads to a shift in fluorescence. This principle is used, for example, to measure viable bacterial cells. In this work, a real-time thermal cycler was used to cultivate bacteria and measure the change in fluorescence during bacterial growth to determine the generation time of different bacterial strains. The ability of the real-time diagram enables a significant simplification and increase in cost efficiency in the generation of bacterial growth curves. It was also possible to monitor the susceptibility of bacteria to antibiotic resistance, which opened another important application of this technique.

中文翻译：

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使用实时热循环仪绘制细菌生长图

生长曲线的生成是确定细菌菌株特性的常用方法。这样的性质可以是浓缩肉汤中的生长速率或对抗生素的抗性。富集方法是枚举细菌和确定生长特性的金标准。较新的方法可以使用在细菌生长过程中被代谢的试剂，从而导致荧光变化。该原理例如用于测量活细菌细胞。在这项工作中，使用实时热循环仪培养细菌并测量细菌生长过程中荧光的变化，以确定不同细菌菌株的产生时间。实时图表的功能可以显着简化细菌生长曲线，并提高成本效率。