The choice between capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) is not easy and depends on many factors. An attempt to facilitate this choice is this work, in which both techniques have been confronted on the basis of the RGB model, offering a transparent and pictorial way to compare individual parameters as well as the overall analytical potential of each tool. To ensure the universal nature of the comparison, a simple in composition and chemically diverse model sample was used, accompanied by the data processing method reducing the potential impact of analyte selection. Moreover, permanent coating of the inner surface of the capillary and addition of a surfactant to the separation buffer were considered as the additional factors that may affect the assessment of the CE technique. The presented analysis can be valuable in any discussions about the intrinsic advantages and disadvantages of CE and HPLC, and divagations on how they affect the overall potential and usability of each. We also provide access to the Excel worksheets used for the assessment, which can be easily modified to reevaluate the methods with a different selection of variables, and analyze other possible scenarios.

中文翻译：

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使用 RGB 模型评估和比较毛细管电泳和高效液相色谱的整体分析潜力：我们能找出多少？

在毛细管电泳 (CE) 和高效液相色谱 (HPLC) 之间进行选择并不容易，取决于许多因素。这项工作试图促进这种选择，其中两种技术都在 RGB 模型的基础上进行了挑战，提供了一种透明和图形化的方式来比较各个参数以及每个工具的整体分析潜力。为确保比较的通用性，使用了一个组成简单且化学性质多样的模型样本，并辅以数据处理方法，以减少分析物选择的潜在影响。此外，毛细管内表面的永久性涂层和向分离缓冲液中添加表面活性剂被认为是可能影响 CE 技术评估的其他因素。所呈现的分析在有关 CE 和 HPLC 的内在优点和缺点的任何讨论中都是有价值的，以及它们如何影响各自的整体潜力和可用性的分歧。我们还提供对用于评估的 Excel 工作表的访问，可以轻松修改这些工作表，以使用不同的变量选择重新评估方法，并分析其他可能的情况。