Saccharothrix algeriensis NRRL B-24137 is an actinobacterium isolated from Algerian Saharan soil. This strain has the ability to produce several dithiolopyrrolone antibiotic derivatives depending on the precursors added to the culture medium. This group of antibiotics is known for their potent antimicrobial and anticancer activities. Holomycin is a member of the dithiolopyrrolone group of antibiotics, and has already been isolated from several species of actinobacteria belonging to the genus Streptomyces and also from some Gram-negative bacteria. In this study, holomycin was produced for the first time in the culture broth of a non-Streptomyces actinobacteria. This antibiotic was induced by adding 5 mM of L-cystine as precursor to the semi-synthetic fermentation broth of Sa. algeriensis NRRL B-24137 and then fully identified after HPLC purification. The minimum inhibitory concentrations (MIC) of holomycin were determined against several pathogenic microorganisms, including Escherichia coli ATCC 10536 Klebsiella pneumoniae CIP 82.91, Listeria monocytogenes CIP 82110, Staphylococcus aureus CIP 7625, Aspergillus carbonarius M333, Fusarium culmorum FC1, Candida albicans IPA 200. This antibiotic showed a broad-spectrum antimicrobial activity, inhibiting a variety of Gram-positive and Gram-negative bacteria, and micro-fungi.

中文翻译：

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Saccharothrix algeriensis NRRL B-24137，第一种非链霉菌放线菌，在喂食胱氨酸后产生全霉素

Saccharothrix algeriensis NRRL B-24137 是一种从阿尔及利亚撒哈拉土壤中分离的放线菌。根据添加到培养基中的前体，该菌株能够产生多种二硫代吡咯酮抗生素衍生物。这组抗生素以其有效的抗菌和抗癌活性而闻名。Holomycin 是抗生素二硫并吡咯酮组的成员，并且已经从属于链霉菌属的几种放线菌以及一些革兰氏阴性菌中分离出来。在这项研究中，全霉素首次在非链霉菌放线菌的培养液中产生。这种抗生素是通过向 Sa 的半合成发酵液中添加 5 mM L-胱氨酸作为前体来诱导的。algeriensis NRRL B-24137，然后在 HPLC 纯化后完全鉴定。全霉素的最低抑菌浓度 (MIC) 是针对几种病原微生物测定的，包括大肠杆菌 ATCC 10536 肺炎克雷伯菌 CIP 82.91、单核细胞增生李斯特菌 CIP 82110、金黄色葡萄球菌 CIP 7625、炭黑曲霉 M333、镰刀菌镰刀菌 FC1020抗生素显示出广谱抗菌活性，抑制多种革兰氏阳性菌和革兰氏阴性菌，以及微真菌。