CRISPR/Cas9 genome editing technology is a DNA manipulation tool for trait improvement. This technology has been demonstrated and successfully applied to edit the genome in various species of plants. The delivery of CRISPR/Cas9 components within rigid plant cells is very crucial for high editing efficiency. Here, we insight the strengths and weaknesses of each method of delivery. The mutation efficiency of genome editing may vary and affected by different factors. Out of various factors, the delivery of CRISPR/Cas9 components into cells and genome is vital. The way of delivery defines whether the edited plant is transgenic or transgene-free. In many countries, the transgenic approach of improvement is a significant limitation in the regulatory approval of genetically modified crops. Gene editing provides an opportunity for generating transgene-free edited genome of the plant. Nevertheless, the mode of delivery of the CRISPR/Cas9 component is of crucial importance for genome modification in plants. Different delivery methods such as Agrobacterium-mediated, bombardment or biolistic method, floral-dip, and PEG-mediated protoplast are frequently applied to crops for efficient genome editing. We have reviewed different delivery methods with prons and cons for genome editing in plants. A novel nanoparticle and pollen magnetofection-mediated delivery systems which would be very useful in the near future. Further, the factors affecting editing efficiency, such as the promoter, transformation method, and selection pressure, are discussed in the present review.

中文翻译：

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目前和未来潜在的在植物中传递 CRISPR/Cas9 组件的方法。


CRISPR/Cas9基因组编辑技术是一种用于性状改良的DNA操作工具。该技术已被证明并成功应用于编辑各种植物的基因组。在刚性植物细胞内传递 CRISPR/Cas9 组件对于高编辑效率至关重要。在这里，我们深入了解每种交付方法的优点和缺点。基因组编辑的突变效率可能会有所不同并受到不同因素的影响。出于多种因素的考虑，将 CRISPR/Cas9 组件传递到细胞和基因组中至关重要。传递方式决定了编辑后的植物是转基因植物还是非转基因植物。在许多国家，转基因改良方法是转基因作物监管审批的重大限制。基因编辑提供了生成无转基因的植物编辑基因组的机会。然而，CRISPR/Cas9 组件的传递方式对于植物基因组修饰至关重要。不同的递送方法，例如农杆菌介导的、轰击或生物射弹方法、花浸法和 PEG 介导的原生质体，经常应用于作物以进行有效的基因组编辑。我们回顾了植物基因组编辑的不同递送方法的优缺点。一种新型纳米颗粒和花粉磁转染介导的递送系统在不久的将来将非常有用。此外，本综述还讨论了影响编辑效率的因素，例如启动子、转化方法和选择压力。