In recent years, açai berries ( Euterpe Oleracea M.), goji berries ( Lycium barbarum L.), bilberries ( Vaccinium myrtillus L.), and chia seeds ( Salvia hispanica L.) have increased interest worldwide due to their nutritional value and health benefits. In the present study, SEC-ICP-MS and μ-HPLC-ESI-MS/MS were used for the investigation of cobalt speciation and evaluation of its bioaccessibility in these products. Total cobalt content was determined, and açai berries (0.348 ± 0.042 μg g −1 ) and chia seeds (0.352 ± 0.036 μg g −1 ) were found as the best sources of this element. Different elution profiles of the extracts of examined berries and seeds obtained with the use of ammonium acetate, Tris-HCl, and SDS suggested that cobalt is bound by different bioligands in each biomatrix. The bioaccessibility of cobalt species was evaluated by SEC-ICP-MS. On the chromatograms of extracts obtained after simulation of gastrointestinal digestion, peaks corresponding to low molecular mass (17.00–1.35 kDa) cobalt complexes were observed. In the case of goji berries, their intensities were significantly higher on chromatograms of gastrointestinal than gastric extract. In enzymatic extracts, different forms of vitamin B 12 were identified by μ-HPLC-ESI-MS, including its natural forms—methylcobalamin (Me-Cbl) and adenosylcobalamin (Ado-Cbl).

中文翻译：

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通过质谱技术研究浆果和种子中钴物种的生物可及性

近年来，巴西莓 (Euterpe Oleracea M.)、枸杞 (Lycium barbarum L.)、越橘 (Vaccinium myrtillus L.) 和奇亚籽 (Salvia hispanica L.) 因其营养价值和健康价值而在全球范围内受到越来越多的关注好处。在本研究中，SEC-ICP-MS 和 μ-HPLC-ESI-MS/MS 用于研究钴形态并评估其在这些产品中的生物可及性。测定了总钴含量，发现巴西莓 (0.348 ± 0.042 μg g -1 ) 和奇亚籽 (0.352 ± 0.036 μg g -1 ) 是该元素的最佳来源。使用醋酸铵、Tris-HCl 和 SDS 获得的受检浆果和种子提取物的不同洗脱曲线表明，钴与每个生物基质中的不同生物配体结合。通过 SEC-ICP-MS 评估钴物种的生物可及性。在模拟胃肠消化后获得的提取物的色谱图上，观察到对应于低分子量 (17.00–1.35 kDa) 钴络合物的峰。在枸杞的情况下，它们在胃肠道色谱图上的强度明显高于胃提取物。在酶提取物中，通过 μ-HPLC-ESI-MS 鉴定了不同形式的维生素 B 12，包括其天然形式——甲钴胺 (Me-Cbl) 和腺苷钴胺 (Ado-Cbl)。