We developed simple sequence repeats (SSR) for Eremanthus erythropappus (DC.) MacLeish, an endangered tree species endemic to the Brazilian Savanna and Atlantic Forest biomes, and tested their transferability to two closely related Eremanthus species. Using a genomic library enriched with tandem repeat motifs, we identified 16 primer pairs, and characterized them in two populations. Nine primers amplified the expected size fragments and seven SSRs were polymorphic, providing a total of 38 alleles and an average of 4.22 alleles per marker. The polymorphic information content (PIC) ranged from 0.44 to 0.94 with an average of 0.65. The average observed heterozygosity across all loci varied from 0.61 to 1.00. The observed (HO) and expected (HE) heterozygosity within the two populations varied from 0.65 to 1.00 and from 0.31 to 1.00, respectively. These newly developed SSR markers are a powerful tool for population genetic analyses and may be useful in studies on species ecology, evolution, and taxonomy.

中文翻译：

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开发和表征Eremanthus erythropappus的核微卫星标记及其在相关物种间的转移性。

我们为巴西大草原和大西洋森林生物群系特有的濒危树种Macerish开发了Eremanthus erythropappus（DC。）的简单序列重复（SSR），并测试了它们对两种紧密相关的Eremanthus物种的可移植性。使用富含串联重复基序的基因组文库，我们鉴定了16对引物，并在两个种群中对其进行了表征。九个引物扩增了预期的大小片段，并且七个SSR是多态性的，每个标记总共提供38个等位基因，平均4.22个等位基因。多态信息含量（PIC）范围从0.44到0.94，平均为0.65。在所有基因座上观察到的平均杂合度从0.61到1.00不等。在两个种群中观察到的（HO）和预期的（HE）杂合度分别从0.65至1.00和0.31至1.00不等。