Reuteransucrase-catalytic kinetic modeling and functional characteristics for novel prebiotic gluco-oligomers.,Food & Function

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Reuteransucrase-catalytic kinetic modeling and functional characteristics for novel prebiotic gluco-oligomers.
Food & Function ( IF 5.1 ) Pub Date : 2020-07-07 , DOI: 10.1039/d0fo00225a
Yuqi Yang ₁ , Yajun Ma ₁ , Xiuting Hu ₁ , Steve W Cui ₂ , Tao Zhang ₁ , Ming Miao ₁

Affiliation

This work describes the reuteransucrase-catalyzed reaction and structural characterization as well as in vitro fermentation for the acceptor products of gluco-oligomers from sucrose and maltose. At a low concentration of sucrose, the production of gluco-oligomers was favored, resulting in a relatively large number of acceptor products (DP_3–5). A mathematical model was also proposed to simulate gluco-oligomer production depending on the reaction conditions. The fine structures of major linear gluco-oligomer fractions for a sucrose : maltose ratio of 1 : 1 were assigned as follows: α-D-Glcp-(1→6)-α-D-Glcp-(1→4)-D-Glcp, α-D-Glcp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→4)-D-Glcp, α-D-Glcp-(1→4)-α-D-Glcp-(1→6)-α-D-Glcp-(1→4)-D-Glcp, and α-D-Glcp-(1→6)-α-D-Glcp-(1→4)-α-D-Glcp-(1→6)-α-D-Glcp-(1→4)-D-Glcp, respectively. Compared with dextran and GOS57, the results of fermentation selectivity indicated that gluco-oligomers promoted the proliferation of gut bacteria and total SCFA production with a higher concentration of propionate. These data suggested that the gluco-oligomers synthesized via the reuteransucrase acceptor reaction had a prebiotic effect on gastrointestinal health.

中文翻译：

新型益生元葡萄糖寡聚物的路透核酸酶催化动力学建模和功能特性。

这项工作描述了蔗糖和麦芽糖的葡萄糖寡聚体受体产物的经Reuteranucrase催化的反应和结构表征以及体外发酵。在低蔗糖浓度下，有利于葡萄糖寡聚物的生产，导致相对大量的受体产物（DP _3-5）。还提出了数学模型以根据反应条件模拟葡萄糖-低聚物的产生。蔗糖：麦芽糖比为1：1的主要线性葡萄糖低聚物主要组分的精细结构分配如下：α- D -Glc p-（1→6）-α - D -Glc p-（1→4） - d -Glc p，α-D -Glc p-（1→4）-α - D -Glc p-（1→4）-α - D -Glc p-（1→4）-D -Glc p，α- D -Glc p-（ 1→4）-α - D -Glc p-（1→6）-α - D -Glc p-（1→4）-D -Glc p和α- D -Glc p-（1→6）- α- D -Glc p-（1→4）-α - D -Glc p-（1→6）-α - D -Glc p-（1→4）-D -Glc p，分别。与葡聚糖和GOS57相比，发酵选择性的结果表明，葡萄糖-低聚物以更高的丙酸酯浓度促进肠道细菌的增殖和总SCFA的产生。这些数据表明，通过路透核酸酶受体反应合成的葡萄糖寡聚体对胃肠道健康具有益生元的作用。

更新日期：2020-08-19

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