Osteosarcoma is characterized by high malignancy and high metastasis rate, resulting in high mortality and disability. MiR-663a has been reported in a variety of tumors to promote tumorigenesis. However, miR-663a has not been reported in the pathogenesis of osteosarcoma. Bioinformatics analysis and experiments including real-time quantitative polymerase chain reaction (RT-qPCR), luciferase reporter, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Western blot, RNA immunoprecipitation, and flow cytometry assay were applied to explore the function and mechanism of miR-663a in MG63, U2OS, Saos-2, SF-86, and hFOB1.19 cells. In this study, we found that miR-663a is highly expressed in osteosarcoma. At the same time, we discovered that miR-663a facilitates cell proliferation and migration, whereas suppresses cell apoptosis in osteosarcoma. Through a series of biological experiments, it was found that miR-663a regulates the cellular process in osteosarcoma by modulating the expression of MYL9. In addition, we also found that long noncoding RNA (lncRNA) GAS5 serves as a molecular sponge for miR-663a and regulates the progression of osteosarcoma via the ceRNA mechanism. We uncover that miR-663a promotes osteosarcoma development through targeting MYL9, which was regulated by lncRNA GAS5.

骨肉瘤的特征是高恶性和高转移率，导致高死亡率和致残性。在多种肿瘤中已经报道了MiR-663a以促进肿瘤发生。但是，miR-663a在骨肉瘤的发病机理中尚未见报道。生物信息学分析和实验，包括实时定量聚合酶链反应（RT-qPCR），萤光素酶报道分子，3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四氮唑测定，蛋白质印迹，RNA免疫沉淀和流式细胞仪用于探讨miR-663a在MG63，U2OS，Saos-2，SF-86和hFOB1.19细胞中的功能和机制。在这项研究中，我们发现miR-663a在骨肉瘤中高度表达。同时，我们发现miR-663a促进细胞增殖和迁移，而抑制骨肉瘤中的细胞凋亡。通过一系列生物学实验，发现miR-663a通过调节MYL9的表达来调节骨肉瘤的细胞过程。此外，我们还发现长的非编码RNA（lncRNA）GAS5充当miR-663a的分子海绵，并通过ceRNA机制调节骨肉瘤的进展。我们发现，miR-663a通过靶向由lncRNA GAS5调控的MYL9促进骨肉瘤的发展。