Chemical Probing of Thymine in the TGG/CGG Triad to Explore the Deamination of 5-Methylcytosine in the CGG Repeat.,Biochemistry

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Chemical Probing of Thymine in the TGG/CGG Triad to Explore the Deamination of 5-Methylcytosine in the CGG Repeat.
Biochemistry ( IF 2.9 ) Pub Date : 2020-07-06 , DOI: 10.1021/acs.biochem.0c00333
Yuki Yagi ₁ , Takeshi Yamada ₁ , Kazuhiko Nakatani ₁

Affiliation

The methylation of cytosine in the full mutation of the expanded CGG repeat and subsequent deamination to thymine could be a measure of repeat instability. We report the synthesis of NCD-Bpy, which binds to the TGG/CGG site in the repeat hairpin. NCD-Bpy forces the thymine in the TGG/CGG site to flip out from the π-stack, recruits osmium tetroxide in the vicinity of the flipped-out T, and oxidizes the T. The piperidine-induced cleavage band successfully determined the position of the T in the expanded CGG repeat.

中文翻译：

在TGG / CGG三联体中进行胸腺嘧啶的化学探测，以探索CGG重复序列中5-甲基胞嘧啶的脱氨基反应。

扩展的CGG重复序列的完全突变以及随后的胸腺嘧啶脱氨反应中胞嘧啶的甲基化可能是重复序列不稳定的一种度量。我们报告了NCD-Bpy的合成，其与重复发夹中的TGG / CGG位点结合。NCD-Bpy迫使TGG / CGG位点的胸腺嘧啶从π堆栈中翻转出来，在翻转的T附近募集四氧化，并氧化T。哌啶诱导的切割带成功地确定了扩展的CGG重复序列中的T。

更新日期：2020-07-28

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