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The Benefits of Going Small: Nanostructures for Mammalian Cell Transfection.
ACS Nano ( IF 15.8 ) Pub Date : 2020-07-06 , DOI: 10.1021/acsnano.0c04624
Andy Tay 1
Affiliation  

Nanostructures, with their localized interactions with mammalian cells, can offer better efficiency and lower cell perturbation than conventional viral, biochemical, and electroporation transfection techniques. In this Perspective, I describe the different stages of transfection and provide a comparison of transfection techniques based on their mechanisms. Focusing on specific aims of transfection, I also illustrate how recent developments in high-aspect-ratio nanostructures have endowed them with properties that are superior to existing viral, biochemical, and electroporation methods as a versatile technique to deliver a variety of cargoes and to interface with different mammalian cell types for biomedical applications. Finally, I describe the challenges associated with transfection that need to be overcome to enhance cargo delivery efficiency and clinical translation.

中文翻译:

变小的好处:用于哺乳动物细胞转染的纳米结构。

与传统的病毒,生化和电穿孔转染技术相比,纳米结构及其与哺乳动物细胞的局部相互作用可以提供更高的效率和更低的细胞扰动。在此“观点”中,我描述了转染的不同阶段,并根据其机制对转染技术进行了比较。着重于转染的特定目标,我还将说明高纵横比纳米结构的最新发展如何赋予它们优于现有病毒,生化和电穿孔方法的特性,以作为运送各种货物和界面的通用技术具有不同哺乳动物细胞类型的生物医学应用。最后,
更新日期:2020-07-28
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