The objective of the study was a comparative analysis of the antihemolytic activity against two Staphylococcus aureus strains (8325-4 and NCTC 5655) as well as α-hemolysin and of the membrane modifying action of four hydrolysable tannins with different molecular mass and flexibility: 3,6-bis-O-di-O-galloyl-1,2,4-tri-O-galloyl-β-d-glucose (T1), 1,2,3,4,5-penta-O-galloyl-β-d-glucose (T2), 3-O-galloyl-1,2-valoneoyl-β-d-glucose (T3) and 1,2-di-O-galloyl-4,6-valoneoyl-β-d-glucose (T4). We showed that all the compounds studied manifested antihemolytic effects in the range of 5–50 µM concentrations. However, the degree of the reduction of hemolysis by the investigated tannins was not uniform. A valoneoyl group—containing compounds (T3 and T4) were less active. Inhibition of the hemolysis induced by α-hemolysin was also noticed on preincubated with the tannins and subsequently washed erythrocytes. In this case the efficiency again depended on the tannin structure and could be represented by the following order: T1 > T2 > T4 > T3. We also found a relationship between the degree of antihemolytic activity of the tannins studied and their capacity to increase the ordering parameter of the erythrocyte membrane outer layer and to change zeta potential. Overall, our study showed a potential of the T1 and T2 tannins as anti-virulence agents. The results of this study using tannins with different combinations of molecular mass and flexibility shed additional light on the role of tannin structure in activity manifestation.

该研究的目的是对两种金黄色葡萄球菌菌株（8325-4和NCTC 5655）以及α-溶血素的抗溶血活性以及四种具有不同分子量和柔韧性的可水解单宁的膜修饰作用进行比较分析：3 ，6 -双- ø -二ö -galloyl-1,2,4-三- ö -galloyl- β - d -葡萄糖（T1），1,2,3,4,5-五- ö -galloyl- β - d -葡萄糖（T2），3- Ô -galloyl -1,2- valoneoyl- β - d -葡萄糖（T3）和1,2-二- ö -galloyl -4,6- valoneoyl- β -d-葡萄糖（T4）。我们表明，所有研究的化合物在5–50 µM浓度范围内均表现出抗溶血作用。但是，所研究的单宁对溶血作用的降低程度并不统一。含瓦龙酰基的化合物（T3和T4）的活性较低。在与单宁酸和随后洗涤的红细胞预孵育时，也注意到了α-溶血素诱导的溶血作用的抑制。在这种情况下，效率再次取决于单宁结构，并且可以由以下顺序表示：T1> T2> T4> T3。我们还发现所研究的单宁的抗溶血活性程度与它们增加红细胞膜外层的有序参数和改变zeta电位之间的关系。总体，我们的研究表明T1和T2单宁具有抗毒作用。这项使用分子量和柔韧性不同组合的单宁的研究结果进一步揭示了单宁结构在活性表现中的作用。