Abstract

The aim of this study was to detect SNPs in myostatin (MSTN) gene of four goat breeds, and analyze the correlation of these markers on body measurement traits in the Dazu black goat breed. In total, twenty polymorphic sites were found in one hundred forty-eight individuals, and all SNPs were distributed in introns 1 and 2, except g. 425 C > T, which was found in the regulatory region. Three SNPs (g. 2732 C > T, g. 2752 G > A and g. 4552 A > C) were polymorphic in all four breeds. None of the tag SNPs (g. 425 C > T, g. 1583 A > G, 2732 C > T, g. 4552 A > C and g. 5167 C > T) were significantly correlated with body measurement traits (p > 0.05) in the Dazu black goat. However, individuals with genotype combination 3 (GtC 3) of tag SNPs had higher birth weight and weaning weight than individuals with the other genotype combinations (p < 0.05). Moreover, the genotype combination 4 (GtC 4) was significantly associated with body length and height at the age of 2 months (p < 0.05), and genotype combination 13 (GtC 13) was significantly correlated with body height at 6 months (p < 0.05). Briefly, the combined tag SNP markers might be useful for goat marker-associated selective breeding.

摘要

本研究的目的是检测四个山羊品种肌肉生长抑制素（MSTN）基因中的SNP ，并分析这些标记与大足黑山羊品种体测量性状的相关性。总共在 148 个个体中发现了 20 个多态位点，所有 SNP 都分布在内含子 1 和 2 中，除了 g。425 C > T，在调控区发现。三个 SNP（g. 2732 C > T、g. 2752 G > A 和 g. 4552 A > C）在所有四个品种中都是多态的。没有一个标签 SNP（g. 425 C > T、g. 1583 A > G、2732 C > T、g. 4552 A > C 和 g. 5167 C > T）与身体测量特征显着相关（p > 0.05) 在大足黑山羊。然而，具有标签 SNP 的基因型组合 3 (GtC 3) 的个体比具有其他基因型组合的个体具有更高的出生体重和断奶体重 ( p  < 0.05)。此外，基因型组合 4 (GtC 4) 与 2 个月时的体长和身高显着相关 ( p  < 0.05)，基因型组合 13 (GtC 13) 与 6 个月时的体高显着相关 ( p  < 0.05）。简而言之，组合标签 SNP 标记可能对山羊标记相关的选择性育种有用。