Many newly identified solute carriers (SLCs) and putative transporters have the possibility to be intricately involved in glucose metabolism. Here we show that many transporters of this type display a high degree of regulation at both mRNA and protein level following no or low glucose availability in mouse cortex cultures. We show that this is also the case in Drosophila melanogaster subjected to starvation or diets with different sugar content. Interestingly, re-introduction of glucose to media, or refeeding flies, normalized the gene expression of a number of the targets, indicating a fast and highly dynamic control. Our findings demonstrate high conservation of these transporters and how dependent both cell cultures and organisms are on gene and protein regulation during metabolic fluctuations. Several transporter genes were regulated simultaneously maybe to initiate alternative metabolic pathways as a response to low glucose levels, both in the cell cultures and in D. melanogaster. Our results display that newly identified SLCs of Major Facilitator Superfamily type, as well as the putative transporters included in our study, are regulated by glucose availability and could be involved in several cellular aspects dependent of glucose and/or its metabolites. Recently, a correlation between dysregulation of glucose in the central nervous system and numerous diseases such as obesity, type 2 diabetes mellitus as well as neurological disease such as Alzheimer’s and Parkinson’s diseases indicate a complex regulation and fine tuning of glucose levels in the brain. The fact that almost one third of transporters and transporter-related proteins remain orphans with unknown or contradictive substrate profile, location and function, pinpoint the need for further research about them to fully understand their mechanistic role and their impact on cellular metabolism.

许多新近鉴定出的溶质载体（SLC）和推定的转运蛋白有可能复杂地参与葡萄糖代谢。在这里，我们显示，在小鼠皮层培养物中没有或有低葡萄糖利用率之后，这种类型的许多转运蛋白在mRNA和蛋白质水平上都显示出高度的调节作用。我们证明在以下情况中也是如此果蝇遭受饥饿或含糖量不同的饮食。有趣的是，将葡萄糖重新引入培养基或重新喂食果蝇可使许多靶标的基因表达正常化，这表明控制是快速而高度动态的。我们的发现证明了这些转运蛋白的高度保守性以及细胞培养物和生物体在代谢波动期间如何依赖基因和蛋白质调节。同时调节了几种转运蛋白基因，可能在细胞培养物中和低水平时启动了新的代谢途径，作为对低葡萄糖水平的反应。D.黑色素瘤我们的结果显示，新鉴定出的主要促进者超家族类型的SLC以及我们研究中包括的推定转运蛋白，均受葡萄糖利用率的调节，并且可能涉及葡萄糖和/或其代谢产物的几个细胞方面。最近，中枢神经系统中的葡萄糖失调与多种疾病（例如肥胖症，2型糖尿病）以及神经系统疾病（例如阿尔茨海默氏病和帕金森氏病）之间存在相关性，这表明大脑中葡萄糖水平的调节和微调非常复杂。几乎三分之一的转运蛋白和与转运蛋白相关的蛋白质仍然是孤儿，其底物特征，位置和功能未知或相互矛盾，