Investigation of glucose utilization during an infection is central to the study of energy metabolism. The heavy utilization of glucose by the malaria parasite, and the consequences of this process, have been investigated extensively. However, host glucose utilization during early infection has not been explored to date. In a first attempt, this article investigates the changes in the host glucose utilization in Balb/c mice infected with Plasmodium berghei ANKA using ¹³C-labeled glucose infusion followed by NMR spectroscopy. The results suggested significant alterations of liver, brain and red blood cell (RBC) glucose utilization during early infection when the parasitemia was <1%. At the pathway level, we observed a decrease in the shunt metabolite 2,3-bisphosphoglycerate in the RBCs. Glycolysis and pathways associated with it, along with fatty acid unsaturation, were altered in the liver. Significant changes were observed in the central carbon metabolic pathways in the brain. These results have implications in understanding the host physiology during early infection and pave the way for detailed flux analysis of the proposed perturbed pathways.

中文翻译：

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代谢组学观察到的疟疾感染的小鼠红细胞，肝和脑中葡萄糖利用的早期扰动。

感染过程中葡萄糖利用的研究是能量代谢研究的核心。疟疾寄生虫大量利用葡萄糖以及该过程的后果已得到广泛研究。然而，迄今为止尚未探索早期感染期间宿主葡萄糖的利用。在首次尝试中，本文使用^{13种方法研究}感染了伯氏疟原虫ANKA的Balb / c小鼠体内宿主葡萄糖利用的变化C标记葡萄糖输注，然后进行NMR光谱分析。结果表明，当寄生虫血症＜1％时，早期感染期间肝，脑和红细胞（RBC）葡萄糖利用率发生了显着变化。在途径水平上，我们观察到红细胞中分流代谢物2,3-双磷酸甘油酸酯的减少。肝中糖酵解和与之相关的途径以及脂肪酸不饱和度被改变。观察到大脑中部碳代谢途径的显着变化。这些结果对理解早期感染过程中的宿主生理有重要意义，并为拟议的扰动途径进行详细的通量分析铺平了道路。