Purkinje cells, the principal neurons of cerebellar computations, are believed to comprise a uniform neuronal population of cells, each with similar functional properties. Here, we show an undiscovered heterogeneity of adult zebrafish Purkinje cells, revealing the existence of anatomically and functionally distinct cell types. Dual patch-clamp recordings showed that the cerebellar circuit contains all Purkinje cell types that cross-communicate extensively using chemical and electrical synapses. Further activation of spinal central pattern generators (CPGs) revealed unique phase-locked activity from each Purkinje cell type during the locomotor cycle. Thus, we show intricately organized Purkinje cell networks in the adult zebrafish cerebellum that encode the locomotion rhythm differentially, and we suggest that these organizational properties may also apply to other cerebellar functions.

小脑计算的主要神经元浦肯野细胞被认为包含统一的神经元细胞群，每个细胞具有相似的功能特性。在这里，我们显示了成年斑马鱼浦肯野细胞的未发现的异质性，揭示了解剖和功能上不同的细胞类型的存在。双重膜片钳记录显示，小脑回路包含所有浦肯野细胞类型，这些类型的浦肯野细胞通过化学和电突触广泛交叉交流。脊髓中央模式发生器（CPG）的进一步激活揭示了运动周期中每种浦肯野细胞类型的独特锁相活性。因此，我们在成年斑马鱼小脑中显示了复杂组织的Purkinje细胞网络，它们以不同的方式编码运动节奏，