A series of DNA markers for various agronomic traits may accelerate the success of marker‐assisted selection in practical plant breeding programmes. Here, we developed DNA markers for the blast resistance gene Pi‐cd. In this study, we examined the effects of the Pi‐cd locus on not only blast resistance but also agronomic traits in agriculture. We developed three pyramiding lines (PLs) coupling Pi‐cd with three blast resistance genes, pi21, Pi35 and Pi39. The effect of Pi‐cd on blast resistance was dependent on the coupled resistance genes. Then, we evaluated the effects of Pi‐cd on 13 agronomic traits. Amylose content and 1,000‐grain weight showed significant differences between the PLs and current commercial varieties, which had no negative effects on agronomic trait values. Furthermore, we investigated the distribution of genotype for the Pi‐cd locus among varieties of upland rice. The KT genotype specific to rice blast resistance may be predominant in the varieties. The results suggested that Pi‐cd has the potential to be useful for improving blast resistance in rice breeding programmes.

中文翻译：

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稻瘟病抗性基因Pi-cd在水稻（Oryza sativa）育种计划中的作用

一系列针对各种农艺性状的DNA标记可能会加速实际植物育种计划中标记辅助选择的成功。在这里，我们开发了抗爆炸基因Pi-cd的DNA标记。在这项研究中，我们研究了Pi-cd基因座不仅对抗瘟性，而且对农业的农艺性状都有影响。我们开发了将Pi-cd与三个抗爆炸基因pi21，Pi35和Pi39偶联的三个金字塔线（PL）。的效果丕CD上稻瘟病抗性依赖于耦合抗性的基因。然后，我们评估了Pi-cd的效果13个农艺性状。直链淀粉含量和1,000粒重显示PL与当前商业品种之间存在显着差异，对农艺性状值没有负面影响。此外，我们研究了旱稻品种Pi-cd基因座的基因型分布。水稻抗稻瘟病特异的KT基因型可能是主要品种。结果表明，Pi-cd具有提高水稻育种程序抗稻瘟病性的潜力。