Enhanced chondrogenesis in a coculture system with genetically manipulated dedifferentiated chondrocytes and ATDC5 cells.,Biotechnology and Bioengineering

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Enhanced chondrogenesis in a coculture system with genetically manipulated dedifferentiated chondrocytes and ATDC5 cells.
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-07-07 , DOI: 10.1002/bit.27482
Yongchang Yao _{1,

2} , Tingshuai Zhang _{1,

3} , Hanzheng Chen _{1,

2} , Shicong Zheng _{1,

2} , Yi Chen _{1,

2} , Shujiang Zhang _{1,

2}

Affiliation

Articular cartilage repair after injury is a great challenge worldwide due to its nerveless and avascular features. Tissue engineering is proposed as a promising alternative for cartilage regeneration. In this study, an adenoviral vector carrying the transforming growth factor‐β3 (TGF‐β3) gene was constructed and introduced into dedifferentiated chondrocytes, which were then cocultured with ATDC5 cells in an alginate hydrogel system. The results showed that the experimental groups exhibited better cell viability and higher levels of cartilage‐related genes than the control groups. In this coculture system, the chondrogenic differentiation of ATDC5 cells was effectively induced by TGF‐β3 and other latent cytokines that were produced by the transfected chondrocytes. Thus, this method can avoid the degradation of exogenous TGF‐β3, and it can protect ATDC5 cells during virus transfection to maintain cell viability and chondrogenic differentiation capability. Taken together, this study provides fresh insights for applying this genetically manipulated coculture system to cartilage repair in the future.

中文翻译：

在具有基因操作的去分化软骨细胞和 ATDC5 细胞的共培养系统中增强软骨形成。

由于其无神经和无血管特征，损伤后的关节软骨修复在世界范围内是一个巨大的挑战。组织工程被提议作为软骨再生的有前途的替代方案。在这项研究中，构建了携带转化生长因子-β3（TGF-β3）基因的腺病毒载体并将其导入去分化的软骨细胞，然后在藻酸盐水凝胶系统中与 ATDC5 细胞共培养。结果表明，与对照组相比，实验组表现出更好的细胞活力和更高水平的软骨相关基因。在该共培养系统中，转染软骨细胞产生的 TGF-β3 和其他潜伏细胞因子可有效诱导 ATDC5 细胞的软骨分化。因此，该方法可以避免外源性 TGF-β3 的降解，在病毒转染过程中可以保护ATDC5细胞，维持细胞活力和软骨分化能力。总之，这项研究为将来将这种基因操纵的共培养系统应用于软骨修复提供了新的见解。

更新日期：2020-09-12

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