Silica nanoparticle assists determining liver cancer gene sequence on interdigitated electrode surface,Biotechnology and Applied Biochemistry

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Silica nanoparticle assists determining liver cancer gene sequence on interdigitated electrode surface
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-07-06 , DOI: 10.1002/bab.1980
Feifei Song ₁ , Yi Yang ₂ , Subash C B Gopinath _{3,

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Affiliation

A high-performance interdigitated electrode (IDE) biosensing surface was reported here by utilizing self-assembled silica nanoparticle (SiNP). The modified surface was used to evaluate the complementation of hairpin forming region from Mitoxantrone resistance gene 7 (MXR7; liver cancer-related short gene). The conjugated SiNPs on 3-aminopropyl triethoxysilane functionalization were captured with probe sequence on IDE biosensing surface. The physical and chemically modified surface was used to quantify MXR7 and an increment in the current response upon complementation was noticed. Limit of target DNA detection was calculated (1–10 fM) and this label-free detection is at the comparable level to the fluorescent-based sensing. A linear regression was calculated [y = 0.243x − 0.0773; R² = 0.9336] and the sensitivity was 1 fM on the linear range of 1 fM to 10 pM. With the strong attachment of capture DNA on IDE through SiNP, the surface clearly discriminates the specificity (complementary) versus nonspecificity (complete-, single-, and triple-mismatched sequences). This detection strategy helps to determine liver cancer progression and the similar strategy can be followed for other gene sequence complementation.

中文翻译：

二氧化硅纳米粒子有助于确定叉指电极表面上的肝癌基因序列

本文通过利用自组装二氧化硅纳米颗粒 (SiNP) 报道了一种高性能叉指电极 (IDE) 生物传感表面。修饰的表面用于评估来自米托蒽醌抗性基因 7（MXR7；肝癌相关短基因）的发夹形成区的互补性。在 IDE 生物传感表面上用探针序列捕获 3-氨基丙基三乙氧基硅烷官能化上的共轭 SiNP。物理和化学改性的表面用于量化 MXR7，并注意到互补时电流响应的增加。计算了目标 DNA 检测的极限 (1–10 fM)，这种无标记检测与基于荧光的传感处于可比水平。计算线性回归 [ y = 0.243 x − 0.0773;R ² = 0.9336] 并且灵敏度在 1 fM 到 10 pM 的线性范围内为 1 fM。由于捕获 DNA 通过 SiNP 牢固附着在 IDE 上，表面可以清楚地区分特异性（互补）与非特异性（完整、单一和三重错配序列）。这种检测策略有助于确定肝癌的进展，其他基因序列互补也可以遵循类似的策略。

更新日期：2020-07-06

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