Protein tyrosine phosphatase, non-receptor type 14 (PTPN14) exerts a profound effect in the progression of multiple malignant tumors. However, whether PTPN14 plays a role in prostate cancer has not been well investigated. Herein, we evaluated the function and potential underlying mechanism of PTPN14 in prostate cancer. Decreased PTPN14 expression was detected in prostate cancer, and restoration of PTPN14 expression in prostate cancer cells inhibited the proliferative and invasive potential. Mechanistically, PTPN14 increased the phosphorylation of Yes-associated protein (YAP) by activation of large tumor suppressor 1 (LATS1), an action that resulted in a significant reduction in YAP-mediated transcriptional activity. Inactivation of YAP by its inhibitor markedly abrogated the PTPN14-knockdown-induced promotion effect on prostate cancer cell proliferation and invasion. Notably, PTPN14 up-regulation also exerted a remarkable suppressive impact on tumorigenesis of prostate cancer in vivo. Taken together, the study reveals a tumor-inhibition role of PTPN14 that represses the proliferation and invasion of prostate cancer by down-regulating YAP activation.

蛋白质酪氨酸磷酸酶（非受体14型）（PTPN14）在多种恶性肿瘤的进展中起着深远的作用。然而，PTPN14是否在前列腺癌中起作用尚未得到很好的研究。在本文中，我们评估了PTPN14在前列腺癌中的功能和潜在的潜在机制。在前列腺癌中检测到PTPN14表达降低，并且在前列腺癌细胞中恢复PTPN14表达抑制了增殖和侵袭潜能。从机制上讲，PTPN14通过激活大型肿瘤抑制因子1（LATS1）来增强Yes相关蛋白（YAP）的磷酸化，这一作用导致YAP介导的转录活性显着降低。通过其抑制剂使YAP失活显着消除了PTPN14敲低诱导的对前列腺癌细胞增殖和侵袭的促进作用。值得注意的是，PTPN14的上调也对前列腺癌的发生产生了显着的抑制作用。体内。两者合计，这项研究揭示了PTPN14的肿瘤抑制作用，它通过下调YAP激活来抑制前列腺癌的增殖和侵袭。