Multiple C2 and Transmembrane Domain Proteins (MCTPs) are putative calcium sensors. Proteins that contain C2 domains play essential roles in membrane trafficking and exocytosis; however, MCTPs functions in neurotransmitter release are not known. Here we report that in C. elegans mctp-1 is under the control of two promoters - one active in the nervous system and the second in the spermatheca. We generated and characterized a loss of function amt1 mutant and compared it to a previously published loss of function mutant (av112). Loss of mctp-1 function causes defects in egg-laying, crawling velocity, and thrashing rates. Both amt1 and av112 mutants are hyposensitive to the acetylcholinesterase blocker aldicarb, suggesting that MCTP-1 may play a role in synaptic vesicle release.

多个 C2 和跨膜结构域蛋白 (MCTP) 是推定的钙传感器。含有 C2 结构域的蛋白质在膜运输和胞吐作用中起着重要作用；然而，MCTPs 在神经递质释放中的功能尚不清楚。在这里我们报告，在秀丽隐杆线虫中，mctp-1受两个启动子的控制——一个在神经系统中活跃，第二个在受精囊中。我们生成并表征了功能缺失突变体amt1并将其与先前公布的功能缺失突变体 ( av112) 进行了比较。mctp-1功能的丧失会导致产蛋、爬行速度和颠簸率出现缺陷。无论AMT1和av112 突变体对乙酰胆碱酯酶阻滞剂涕灭威不敏感，表明 MCTP-1 可能在突触小泡释放中起作用。