The aim of this experiment was to investigate the effects of reducing dietary fishmeal (FM) with yeast culture (SYC) supplementation on growth, immune response, intestinal microbiota, intestinal morphology, and disease resistance of Litopenaeus vannamei. A total of 480 shrimps with an average initial body weight of 0.35 ± 0.002 g were randomly distributed into twelve tanks. Three isonitrogenous (40.00 crude protein) and isolipidic (8.00 crude lipids) diets with yeast culture supplementing fishmeal were formulated. The groups were divided into two (2) namely control group and experimental groups. The formulations of the groups were control (0 %, without yeast culture) and the experiment groups (SYC) [(1 % of yeast culture), and (2 % of yeast culture)]. Each diet was delivered in four replicate per treatment group. The results indicate significant improvement on the growth indices (specific growth rate, weight gain rate, survival rate and lower feed conversion ratio) with yeast culture treatment group after 56 days feeding trials (P < 0.05). Total hemolymph protein, superoxide dismutase, catalase, alkaline phosphatase, acid phosphatase, lysozyme and phenoxidase were enhanced but low aspartate aminotransferase, alanine aminotransferase, and glucose were observed in shrimp fed yeast culture diets (P < 0.05). The SYC groups showed insignificant differences in hemolymph cholesterol and triglyceride. Proteobacteria, Bacteroidetes, and Actinobacteria were the dominant bacteria found in all the SYC groups. At the genus level, Vibrio was significantly decreased (P < 0.05) in 2 % yeast culture diets supplemented group whereas the beneficial bacteria Pseudoalteromonas was significantly enhanced. Moreover, intestinal villus length and width in shrimps fed yeast culture diets were improved (P < 0.05). Dietary yeast culture supplementation can improve growth, intestinal health, immune response, and resistance against Vibrio harveyi infections in L. vannamei.

本实验的目的是研究补充酵母培养物（SYC）减少饮食中的鱼粉（FM）对凡纳滨对虾的生长，免疫反应，肠道菌群，肠道形态和抗病性的影响。将总共​​480只平均初始体重为0.35±0.002 g的虾随机分配到十二个池中。配制了三种等氮饲料（40.00粗蛋白）和等脂饮食（8.00粗脂质），辅以酵母培养物补充鱼粉。各组分为两（2）组，即对照组和实验组。各组的配方为对照组（0％，无酵母培养物）和实验组（SYC）[（（1％酵母培养物）和（2％酵母培养物）]。每个饮食组每个饮食一式四份。结果表明，经过56天的饲喂试验后，酵母培养处理组的生长指数（比生长速率，增重率，存活率和较低的饲料转化率）有了显着改善（P <0.05）。总血淋巴蛋白，超氧化物歧化酶，虾饲料酵母培养日粮中过氧化氢酶，碱性磷酸酶，酸性磷酸酶，溶菌酶和苯氧合酶得到增强，但观察到低天冬氨酸转氨酶，丙氨酸转氨酶和葡萄糖（P <0.05）。SYC组的血淋巴胆固醇和甘油三酯差异不明显。蛋白质细菌，拟杆菌和放线菌是在所有SYC组中发现的优势细菌。在属上弧菌在补充组而有益细菌2％酵母培养物的饮食降低显著（P <0.05）假交替单胞物显著增强。此外，饲喂酵母培养饲料的虾的肠绒毛长度和宽度均得到改善（P <0.05）。膳食酵母培养补充剂可改善L的生长，肠道健康，免疫反应以及对哈维弧菌感染的抵抗力。南美白对虾。