In this work, poly(ethylene glycol)-b-poly[3-acrylamidophenylboronic acid-co-styrene] (PEG-b-P(PBA-co-St) has been firstly synthesized for loading of insulin to form insulin-loaded micelles. Insulin-loaded micelles (ILM) and epidermal growth factor (EGF) are further embedded into the composite hydrogels that can be rapidly gelled by mixing of oxidized hyaluronic acid (OHA) and succinyl chitosan (SCS). Then, the morphology, rheology, degradation, swelling and cytotoxicity properties of the as-prepared composite hydrogels are further investigated to evaluate their physical properties and biocompatibility of as the wound dressing. The as-prepared composite hydrogels show the excellent cell compatibility and low toxicity. To evaluate the wound healing ability of as-prepared composite hydrogels, the tests of wound healing in vivo are conducted on streptozotocin-induced rat models. And the as-prepared composite hydrogels with ILM and EGF show an excellent wound healing performance for promotion of fibroblast proliferation and tissue internal structure integrity, as well as the deposition of collagen and myofibrils. These results suggest that the as-prepared composite hydrogels with loading of ILM and EGF could be a promising candidate for wound healing applications.

在这项工作中，聚（乙二醇）-b-聚[3-丙烯酰胺基苯基硼酸-共-苯乙烯]（PEG- b - P（PBA- co-St）已经被首先合成用于负载胰岛素以形成负载胰岛素的胶束。载有胰岛素的胶束（ILM）和表皮生长因子（EGF）进一步嵌入到复合水凝胶中，可以通过混合氧化透明质酸（OHA）和琥珀酰壳聚糖（SCS）使其迅速胶凝。然后，进一步研究了所制备的复合水凝胶的形态，流变，降解，溶胀和细胞毒性，以评估其物理特性和作为伤口敷料的生物相容性。所制备的复合水凝胶显示出优异的细胞相容性和低毒性。为了评估制备的复合水凝胶的伤口愈合能力，体内伤口愈合测试在链脲佐菌素诱导的大鼠模型上进行。制备的具有ILM和EGF的复合水凝胶具有出色的伤口愈合性能，可促进成纤维细胞增殖和组织内部结构完整性，以及胶原蛋白和肌原纤维的沉积。这些结果表明，负载ILM和EGF的复合水凝胶可能是伤口愈合应用的有希望的候选者。