Nanocomposite scaffolds were fabricated from poly (ε-caprolactone) (PCL), Poly (2-hydroxyethylmethacrylate) (PHEMA), and Apacite (Apatite-calcite) nanostructures (15 and 25 wt%). The nanoscale structure, physical and chemical properties, mechanical properties, hydrophilic behavior, degradability and osteogenic properties of the fabricated scaffolds were evaluated. The results showed that the mechanical strength, degradation, wetting ability, and mechanical strength of PCL-PHEMA scaffolds significantly increases upon inclusion of Apacite nanoparticles up to 25 wt%. Accordingly, the best mechanical values (E ~ 7.109 MPa and σ ~ 0.414 MPa) and highest degradability (32% within 96 h) were recorded for PCL-PHEMA scaffolds containing 25 wt% of Apacite. Furthermore, highest porosity and roughness were observed in the PCL-PHEMA/25% Apacite as a result of the Apacite nanoparticles inclusion. There was no cytotoxicity recorded for the fabricated scaffolds based on the results obtained from MTT assay and acridine orange staining. Alkaline phosphatase activity, calcium content quantification, Van Kossa staining, FESEM and real time PCR tests confirmed the biomineralization, and the differentiation potential of the nanocomposite scaffolds. Overall, the 3D structure, optimum porosity and balanced dissolution rate of PCL-PHEMA/25% Apacite providing a balanced microenvironment resulted in improved cell adhesion, cell behavior, and replication, as well as osteogenic induction of human bone-marrow-derived mesenchymal stem cells (hBM-MSCs).

纳米复合支架由聚（ε-己内酯）（PCL），聚（甲基丙烯酸2-羟乙酯）（PHEMA）和磷灰石（磷灰石-方解石）纳米结构（15和25 wt％）制成。评价了制备的支架的纳米级结构，物理和化学性质，机械性质，亲水行为，可降解性和成骨性质。结果显示，当包含高达25 wt％的磷灰石纳米颗粒时，PCL-PHEMA支架的机械强度，降解，润湿能力和机械强度显着提高。因此，对于含25％（重量）磷灰石的PCL-PHEMA支架，记录了最佳的机械值（E〜7.109 MPa和σ〜0.414 MPa）和最高的可降解性（96小时内为32％）。此外，由于包含了磷灰石纳米颗粒，在PCL-PHEMA / 25％磷灰石中观察到最高的孔隙率和粗糙度。基于从MTT测定和a啶橙染色获得的结果，没有记录到所制备的支架的细胞毒性。碱性磷酸酶活性，钙含量定量，Van Kossa染色，FESEM和实时PCR测试证实了纳米复合支架的生物矿化和分化潜力。总体而言，PCL-PHEMA / 25％磷灰石的3D结构，最佳孔隙率和平衡的溶出度提供了平衡的微环境，从而改善了细胞的粘附，细胞行为和复制，并诱导了人骨髓间充质干的成骨诱导细胞（hBM-MSC）。基于从MTT测定和a啶橙染色获得的结果，没有记录到所制备的支架的细胞毒性。碱性磷酸酶活性，钙含量定量，Van Kossa染色，FESEM和实时PCR测试证实了纳米复合支架的生物矿化和分化潜力。总体而言，PCL-PHEMA / 25％磷灰石的3D结构，最佳孔隙率和平衡的溶出度提供了平衡的微环境，从而改善了细胞的粘附，细胞行为和复制，并诱导了人骨髓间充质干的成骨诱导细胞（hBM-MSC）。基于从MTT测定和a啶橙染色获得的结果，没有记录到所制备的支架的细胞毒性。碱性磷酸酶活性，钙含量定量，Van Kossa染色，FESEM和实时PCR测试证实了纳米复合支架的生物矿化和分化潜力。总体而言，PCL-PHEMA / 25％磷灰石的3D结构，最佳孔隙率和平衡的溶出度提供了平衡的微环境，从而改善了细胞的粘附，细胞行为和复制，并诱导了人骨髓间充质干的成骨诱导细胞（hBM-MSC）。FESEM和实时PCR测试确认了纳米复合支架的生物矿化和分化潜力。总体而言，PCL-PHEMA / 25％磷灰石的3D结构，最佳孔隙率和平衡的溶出度提供了平衡的微环境，从而改善了细胞的粘附，细胞行为和复制，并诱导了人骨髓间充质干的成骨诱导细胞（hBM-MSC）。FESEM和实时PCR测试证实了生物矿化作用以及纳米复合支架的分化潜力。总体而言，PCL-PHEMA / 25％磷灰石的3D结构，最佳孔隙率和平衡的溶出度提供了平衡的微环境，从而改善了细胞的粘附，细胞行为和复制，并诱导了人骨髓间充质干的成骨诱导细胞（hBM-MSC）。