Influence of subclinical mastitis and intramammary infection by coagulase-negative staphylococci on the cow milk peptidome.,Journal of Proteomics

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Influence of subclinical mastitis and intramammary infection by coagulase-negative staphylococci on the cow milk peptidome.
Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-07-07 , DOI: 10.1016/j.jprot.2020.103885
Maria Filippa Addis ₁ , Elisa Margherita Maffioli ₂ , Fabrizio Ceciliani ₃ , Gabriella Tedeschi ₂ , Valentina Zamarian ₃ , Francesco Tangorra ₄ , Mariangela Albertini ₃ , Renata Piccinini ₃ , Valerio Bronzo ₃

Affiliation

Coagulase-negative staphylococci (CNS) are the most prevalent microorganisms isolated from cow milk and are associated with subclinical mastitis and persistent increases in the bulk milk somatic cell count (BMSCC) of low BMSCC herds. By combining peptide enrichment, LC-ESI-MS/MS, and statistical analysis, we investigated the influence of subclinical mastitis and CNS infection on the milk peptidome. Quarter milk samples from clinically healthy Holstein cows were subjected to bacteriological culture (BC) and somatic cell counting (SCC) for two consecutive samplings and 28 (including 11 negatives and 17 positives) were selected for peptidomic analysis.

The study identified 1363 different endogenous peptides and highlighted a significant increase of peptides in CNS-positive milk, mainly represented by casein fragments. Milk peptidome changes increased with the SCC, as also demonstrated by protein electrophoresis and densitometry. Peptides significantly different in CNS or CONTROL samples were identified and characterized. Our results indicate that subclinical mastitis by CNS can induce significant changes in the milk peptidome, opening the way to future studies for the identification of a biomarker panel as well as for the understanding of their consequences for the technological and sensorial characteristics of cow milk and dairy products.

Significance

This is the first investigation on the impact of subclinical CNS mastitis on the bovine milk peptidome. The peptide enrichment strategy combined with a highly sensitive MS/MS analysis enabled the compilation of a very large peptide dataset for healthy and mastitic milk. The comparison of CNS and Control samples, also considering SCC classes, highlighted several peptides with potential for understanding milk protein and peptide dynamics in subclinical mastitis, with possible implications for its detection.

中文翻译：

亚临床乳腺炎和凝固酶阴性葡萄球菌对乳内感染的影响。

凝固酶阴性葡萄球菌（CNS）是从牛奶中分离出的最普遍的微生物，与亚临床乳腺炎和低BMSCC牛群的散装牛奶体细胞计数（BMSCC）持续增加有关。通过结合肽浓缩，LC-ESI-MS / MS和统计分析，我们研究了亚临床乳腺炎和中枢神经系统感染对乳肽组的影响。对来自临床健康的荷斯坦奶牛的四分之一牛奶样品进行细菌培养（BC）和体细胞计数（SCC），进行两次连续采样，并选择28种（包括11种阴性和17种阳性）进行肽组分析。

这项研究鉴定了1363种不同的内源肽，并突出显示了CNS阳性乳汁中肽的显着增加，主要由酪蛋白片段代表。牛奶肽组的变化随着SCC的增加而增加，这也通过蛋白质电泳和光密度测定法得到证实。鉴定并鉴定了CNS或CONTROL样品中显着不同的肽。我们的结果表明，中枢神经系统的亚临床乳腺炎可引起牛奶肽组的显着变化，从而为未来的研究奠定了基础，以鉴定生物标志物以及了解其对牛奶和乳制品技术和感官特性的影响产品。