Emerging global danger of multidrug resistant microbes makes it essential to explore new approaches to treat infections. We studied antibacterial and pro-regenerative effects of photodynamic therapy (PDT) performed with water solutions of photodithazine and its complexes with Pluronic F127 and chitosan in rat model of full thickness wound (n = 24) infected by an associated Gram-negative and Gram-positive bacteria culture. Laboratory rats were exposed to PDT 24 and 72 h after the injury. Exudate samples were collected before and after PDT for a microbiological study. Autopsy tissues were excised and fixed in formalin on day 4 of the experiment. Fixed tissues were processed and poured into paraffin. Paraffin sections were stained with hematoxylin and eosin and studied by an experienced pathologist. Microbiological analysis revealed that the photoactivation of photodithazine and its complexes suppressed the associated microflora in vivo and inhibited suppurative inflammation in the wounds. The triple Photodithazine-Pluronic F127-Chitosan system possessed the highest antibacterial activity. The morphological study revealed that PDT with photodithazine polymer complexes accelerated wound healing, promoted restoration of microcirculation, facilitated proliferation of fibroblast and vessels and stimulated collagen synthesis. The Photodithazine-Pluronic F127-Chitosan complex may be successfully applied for PDT to prevent and treat suppurative inflammatory diseases of the skin and soft tissues.

对多药耐药性微生物的全球威胁的兴起使得探索治疗感染的新方法至关重要。我们研究了光动力疗法（PDT）的光动力疗法（PDT）及其与Pluronic F127和壳聚糖的复合物的水溶液在全层伤口（n = 24）被相关的革兰氏阴性和革兰氏阳性细菌培养物感染。实验大鼠在受伤后24和72 h暴露于PDT。在PDT之前和之后收集渗出液样品，以进行微生物学研究。在实验的第4天，将尸体解剖组织切除并固定在福尔马林中。处理固定的组织并倒入石蜡中。石蜡切片用苏木精和曙红染色，并由经验丰富的病理学家进行研究。微生物学分析表明，光二嗪及其配合物的光活化抑制了体内相关的菌群并抑制了伤口的化脓性炎症。三联Photodithazine-Pluronic F127-壳聚糖系统具有最高的抗菌活性。形态学研究表明，PDT与光二嗪聚合物配合物可加速伤口愈合，促进微循环的恢复，促进成纤维细胞和血管的增殖并刺激胶原蛋白的合成。Photodithazine-Pluronic F127-壳聚糖复合物可成功用于PDT，以预防和治疗皮肤和软组织的化脓性炎症性疾病。