Sour rot, caused by Geotrichum citri-aurantii, is a major postharvest disease of citrus，and it causes serious economic losses. In this study, a high-quality genome sequence of G. citri-aurantii was obtained by Single Molecule Real-Time Sequencing (SMRT). Approximately 5.43 Gb of clean data were obtained and a total of 27.94-Mb genomic sequence was mapped to 10 chromosome groups after high-through chromosome conformation capture (Hi-C) assembly. In addition, three polygalacturonase genes which were related to pathogenicity in G. citri-aurantii genome were discovered. And transcriptome data of guazatine-resistance had been analyzed, the results showed that the guazatine-resistance of G. citri-aurantii was related to two ATP-binding cassette (ABC) transporter family genes, six major facilitator superfamily (MFS) transporter family genes and two multidrug and toxic compound extrusion (MATE) transporter family genes. In summary, our research may provide novel insights into the effective control of this pathogen.

柑桔黄腐病是柑桔采后的主要病害，造成严重的经济损失。在这项研究中，通过单分子实时测序（SMRT）获得了高质量的柑橘属植物基因组序列。获得了大约 5.43 Gb 的干净数据，在高通染色体构象捕获 (Hi-C) 组装后，总共 27.94-Mb 的基因组序列被映射到 10 个染色体组。此外，在G. citri-aurantii基因组中还发现了三个与致病性相关的多聚半乳糖醛酸酶基因。并分析了耐瓜扎汀的转录组数据，结果表明G. citri-aurantii的瓜扎汀抗性与两个 ATP 结合盒 (ABC) 转运蛋白家族基因、六个主要促进剂超家族 (MFS) 转运蛋白家族基因和两个多药和有毒化合物挤出 (MATE) 转运蛋白家族基因有关。总之，我们的研究可能为有效控制这种病原体提供新的见解。