Salinity is a major limiting factor in crop production. Exogenous spermidine (spd) effectively ameliorates salt injury, though the underlying molecular mechanism is poorly understood. We have used a suppression subtractive hybridization method to construct a cDNA library that has identified up-regulated genes from rice root under the treatment of spd and salt. Total 175 high-quality ESTs of about 100–500 bp in length with an average size of 200 bp are isolated, clustered and assembled into a collection of 62 unigenes. Gene ontology analysis using the KEGG pathway annotation database has classified the unigenes into 5 main functional categories and 13 subcategories. The transcripts abundance has been validated using Real-Time PCR. We have observed seven different types of post-translational modifications in the DEPs. 44 transmembrane helixes are predicted in 6 DEPs. This above information can be used as first-hand data for dissecting the administrative role of spd during salinity.

盐度是作物生产的主要限制因素。外源性亚精胺 (spd) 可有效改善盐损伤，尽管其潜在的分子机制知之甚少。我们使用抑制消减杂交方法构建了一个 cDNA 文库，该文库在 spd 和盐处理下鉴定了水稻根部的上调基因。总共有 175 个长度约为 100-500 bp、平均大小为 200 bp 的高质量 EST 被分离、聚集并组装成 62 个 unigenes 的集合。使用 KEGG 通路注释数据库的基因本体分析将 unigenes 分为 5 个主要功能类别和 13 个子类别。转录本丰度已使用实时 PCR 进行验证。我们在 DEP 中观察到七种不同类型的翻译后修饰。在 6 个 DEP 中预测了 44 个跨膜螺旋。上述信息可用作剖析 spd 在盐度期间的管理作用的第一手数据。