OBJECTIVE To evaluate the effect of coenzyme Q10 (CoQ10) supplementation on oocyte maturation rates and postmeiotic aneuploidy rates during in vitro maturation (IVM) of human oocytes. DESIGN Clinical laboratory observation. SETTING Hospital and university laboratories. PATIENT(S) Forty-five patients aged ≥38 years and 18 patients aged ≤30 years undergoing in vitro fertilization. INTERVENTION(S) The germinal vesicle-stage oocytes and associated cumulus cells were cultured in IVM media for 24-48 hours with or without 50 μmol/L CoQ10. Oocyte maturation rates were determined based on the presence or absence of the first polar body. Postmeiotic aneuploidies were determined using next-generation sequencing analyses of biopsied polar bodies. MAIN OUTCOME MEASURE(S) Oocyte maturation rates, postmeiotic oocyte aneuploidy rates, and chromosome aneuploidy frequencies. RESULT(S) In women aged 38-46 years, 50 μmol/L CoQ10 significantly increased oocyte maturation rates (82.6% vs. 63.0%; P=.035), reduced oocyte aneuploidy rates (36.8% vs. 65.5%; P=.020), and reduced chromosome aneuploidy frequencies (4.1% vs. 7.0%; P=.012. In women aged ≤30 years, we failed to demonstrate an effect of CoQ10 on oocyte maturation rates or postmeiotic aneuploidies. CONCLUSION(S) CoQ10 supplementation during IVM increased oocyte maturation rates and reduced postmeiotic aneuploidies for older women.

中文翻译：

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辅酶 Q10 补充人卵母细胞体外成熟可减少减数分裂后非整倍体

目的 评估辅酶 Q10 (CoQ10) 补充对人卵母细胞体外成熟 (IVM) 过程中卵母细胞成熟率和减数分裂后非整倍体率的影响。设计 临床实验室观察。设置医院和大学实验室。患者 45 名年龄≥38 岁的患者和 18 名年龄≤30 岁的患者接受体外受精。干预措施 将生发囊泡阶段的卵母细胞和相关卵丘细胞在含有或不含 50 μmol/L CoQ10 的 IVM 培养基中培养 24-48 小时。卵母细胞成熟率是根据第一极体的存在与否来确定的。使用活检极体的下一代测序分析确定减数分裂后的非整倍体。主要结局指标 卵母细胞成熟率、减数分裂后卵母细胞非整倍体率、和染色体非整倍性频率。结果 (S) 在 38-46 岁的女性中，50 μmol/L CoQ10 显着增加卵母细胞成熟率（82.6% 对 63.0%；P=.035），降低卵母细胞非整倍体率（36.8% 对 65.5%；P= .020) 和染色体非整倍性频率降低（4.1% 与 7.0%；P=.012。在 30 岁以下的女性中，我们未能证明辅酶 Q10 对卵母细胞成熟率或减数分裂后非整倍体有影响。结论（S）辅酶 Q10 IVM 期间的补充增加了卵母细胞的成熟率并减少了老年女性的减数分裂后非整倍体。我们未能证明 CoQ10 对卵母细胞成熟率或减数分裂后非整倍体的影响。结论（S）在 IVM 期间补充辅酶 Q10 可增加老年女性的卵母细胞成熟率并减少减数分裂后的非整倍体。我们未能证明 CoQ10 对卵母细胞成熟率或减数分裂后非整倍体的影响。结论（S）在 IVM 期间补充辅酶 Q10 可增加老年女性的卵母细胞成熟率并减少减数分裂后的非整倍体。