The present study explores the effect of ethylene diamine disuccinic acid (EDDS) and gibberellic acid (GA) application on the phytoextraction of copper and zinc ions by Lolium perenne. When Cu was individually applied, accumulation diminished over time with little translocation from roots to shoots. In contrast, Zn accumulation and damage to roots rapidly increased over 3 days with increase in Zn translocation to shoots. Co-application of Zn to Cu amended treatments enhanced Cu concentration in shoots. For the Cu_EDDS application, EDDS significantly increased Cu accumulation and the damage to root increased over time, while gibberellic acid applied with Cu and Zn generally lowered metal uptake and decreased cell membrane damage. The application of EDDS and GA-EDDS, by themselves or with Cu and Zn, lowered transpiration and increased translocation, while GA increased transpiration but decreased translocation. EDDS application typically increased metal ion uptake by causing more cell damage, while GA typically lowered the damage and decreased metal uptake even though the transpiration increased over time and plant growth occurred. Furthermore, the behaviour of metal uptake changed over time and, for some treatments, the short-term and long-term response differed greatly. These results show that EDDS can be successfully used in phytoextraction of both Cu and Zn ions by Lolium perenne while GA can resist damage and protect against plant stress.

本研究探讨了乙二胺二琥珀酸（EDDS）和赤霉素（GA）的应用对黑麦草提取铜和锌离子的影响。当单独施用铜时，随着时间的流逝，积累量逐渐减少，从根到芽几乎没有移位。相反，随着锌向芽的转运增加，锌的积累和对根的损害在三天内迅速增加。锌与铜的联合处理可提高枝条中的铜含量。对于铜_EDDS随着时间的流逝，EDDS显着增加了铜的积累，对根的伤害也增加了，而赤霉素与铜和锌的施用通常会降低金属的吸收并减少细胞膜的伤害。单独使用EDDS和GA-EDDS或与铜和锌一起使用，可减少蒸腾作用并增加易位，而GA可增加蒸腾作用但减少易位。EDDS的使用通常会引起更多的细胞损伤，从而增加金属离子的吸收，而GA则通常会降低损伤，并减少金属的吸收，即使蒸腾作用随时间的增长而增加，并且会发生植物生长。此外，金属吸收的行为会随着时间而改变，对于某些处理，短期和长期反应差异很大。多年生黑麦草，GA可以抵抗伤害并保护植物免受压力。