The specific determination of L-hydroxyproline (Hyp) can serve as a potential indicator for early clinical diagnosis of liver fibrosis. In this work, an integrated strategy based on 4-plex stable isotope labeling derivatization combined with dummy magnetic molecularly imprinted polymers (QSILD-DMMIPs) was developed for specific extraction and rapid determination of Hyp in human serum by ultra high performance liquid chromatography tandem mass spectrometry. A new series of QSILD reagents d0/d1/d2/d3-6-N-methyl-rhodamine 6G-N-hydroxysuccinimidyl formate (d0/d1/d2/d3-MRSF) were designed, synthesized and applied for the high-throughput labeling of Hyp in serum samples. The structural analogue derivative of Hyp with 6-N-ethyl-rhodamine 6G-N-hydroxysuccinimidyl formate (ERSF-Hyp) was synthesized and used as a novel dummy template to prepare DMMIPs. The DMMIPs were well characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), fourier transform infrared spectroscopy (FTIR), Brunner Emmet Teller (BET) measurements, thermogravimetric analysis (TGA), X-ray diffraction (XRD), zeta potential and adsorption experiments. All d0/d1/d2/d3-MRSF-Hyp derivatives were conveniently and specifically adsorbed by DMMIPs in magnetic dispersive solid phase extraction procedure before injection. Method validation results including linearity (0.2-100 ng mL-1), limits of detection and quantitation (0.05 and 0.2 ng mL-1), accuracy, precision, stability, matrix effect and derivatization efficiency were satisfactory. The analytical performances benefited from efficient integration of QSILD and specific DMMIPs extraction. The proposed strategy was successfully applied for Hyp determination in human serum of liver fibrosis patients and healthy controls, which was of great significance to early diagnosis.

中文翻译：

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基于衍生化的磁性假分子印迹聚合物与 4 重稳定同位素标记衍生化策略相结合，用于特异性快速测定人血清中的 L-羟脯氨酸

L-羟脯氨酸（Hyp）的特异性测定可作为肝纤维化早期临床诊断的潜在指标。在这项工作中，开发了一种基于 4 重稳定同位素标记衍生化结合虚拟磁性分子印迹聚合物 (QSILD-DMMIPs) 的集成策略，用于通过超高效液相色谱串联质谱法特异性提取和快速测定人血清中的 Hyp。 . 设计合成了新系列的 QSILD 试剂 d0/d1/d2/d3-6-N-甲基-罗丹明 6G-N-羟基琥珀酰亚胺甲酸酯 (d0/d1/d2/d3-MRSF) 并应用于高通量标记血清样品中的 Hyp。合成了 Hyp 与 6-N-乙基-罗丹明 6G-N-羟基琥珀酰亚胺甲酸酯 (ERSF-Hyp) 的结构类似物衍生物，并用作制备 DMMIPs 的新型虚拟模板。通过扫描电子显微镜 (SEM)、透射电子显微镜 (TEM)、傅里叶变换红外光谱 (FTIR)、Brunner Emmet Teller (BET) 测量、热重分析 (TGA)、X 射线衍射 (XRD)、 zeta 电位和吸附实验。所有 d0/d1/d2/d3-MRSF-Hyp 衍生物都在进样前通过磁分散固相萃取程序方便且特异性地被 DMMIP 吸附。方法验证结果包括线性（0.2-100 ng mL-1）、检测限和定量限（0.05 和0.2 ng mL-1）、准确度、精密度、稳定性、基质效应和衍生化效率均令人满意。分析性能得益于 QSILD 和特定 DMMIP 提取的有效集成。