A field-applicable colorimetric assay for fast detection of notorious explosive triacetone triperoxide (TATP) has been developed through the selective irreversible oxidation of 3, 3′-diaminobenzidine by hydrogen peroxide (HP) liberated during the acidic hydrolysis/degradation of TATP in the presence of MnO 2 nanozymes. The generated HP was detected by probing the absorbance of the product (indamine polymer) of the 3, 3′-diaminobenzidine (DAB) oxidation reaction at 460.0 nm. The UV-Vis measurements provided a linear range from 1.57 to 10.50 mg L −1 TATP with a detection limit of 0.34 mg L −1 . The oxidation of DAB cannot proceed by molecular oxygen, thus it is selectively oxidized by H 2 O 2 ; this prevents false-positive results from laundry detergents (containing O 2 -releasing substances). Moreover, a naked-eye field test was developed, and a fast spot test analyzing time of 5 s was achieved. The selectivity of the assay was checked by analyzing some synthetic samples prepared with a laundry detergent as camouflage. The results of the developed assay revealed quantitative recoveries for TATP whereas the standard nanozyme-based method showed significant false-positive results. Graphical abstract

中文翻译：

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通过纳米酶催化 3, 3'-二氨基联苯胺的不可逆氧化对臭名昭著的爆炸性三过氧化三丙酮进行现场适用的比色测定

通过在 TATP 酸性水解/降解过程中释放的过氧化氢 (HP) 选择性不可逆氧化 3, 3'-二氨基联苯胺，开发了一种适用于现场的比色法，用于快速检测臭名昭著的爆炸性三过氧化三丙酮 (TATP) MnO 2 纳米酶。通过探测 3, 3'-二氨基联苯胺 (DAB) 氧化反应的产物（茚满胺聚合物）在 460.0 nm 处的吸光度来检测生成的 HP。UV-Vis 测量提供了从 1.57 到 10.50 mg L -1 TATP 的线性范围，检测限为 0.34 mg L -1 。DAB的氧化不能通过分子氧进行，因此它被H 2 O 2 选择性氧化；这可以防止洗衣粉（含有 O 2 释放物质）的假阳性结果。此外，还开发了裸眼现场测试，并且实现了 5 秒的快速现场测试分析时间。通过分析一些用洗衣粉作为伪装制备的合成样品来检查测定的选择性。开发的检测结果显示了 TATP 的定量回收率，而基于纳米酶的标准方法显示了显着的假阳性结果。图形概要