Microbes associated with the external and internal anatomy of three commercially available predatory mite species—Phytoseiulus persimilis, Typhlodromips (= Amblyseius) swirskii, and Neoseiulus (= Amblyseius) cucumeris—were examined using light microscopy, confocal laser scanning microscopy and fluorescence in-situ hybridization (FISH). Four microbe morphotypes were observed on external body regions. These included three microfungi-like organisms (named T1, T2 and T3) and rod-shaped bacteria (T4). Morphotypes showed unique distributions on the external body regions and certain microbes were found only on one host species. Microfungi-like T1 were present in all three species whereas T2 and T3 were present in only P. persimilis and T. swirskii, respectively. T1 and T2 microbes were most abundant on the ventral structures of the idiosoma and legs, most frequently associated with coxae, coxal folds, ventrianal shields and epigynal shields. T3 microbes were most abundant on legs and dorsal idiosoma. T4 microbes were less abundant and were attached to epigynal shields of N. cucumeris and T. swirskii. Significant differences in distribution between batches suggest temporal fluctuations in the microbiota of phytoseiids in mass-reared systems. FISH showed bacteria within the alimentary tract, in Malpighian tubules and anal atria. These may aid absorption of excretory products or maintaining gut physiology. We suggest a mechanism by which microbes may be transmitted to offspring and throughout populations. This study aims to improve our knowledge of this poorly understood area and highlights the necessity of understanding the microbiota of Acari.

中文翻译：

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显微镜下分析了三种商业植物科（Acari：Mesostigmata）的微生物群。

与三种市售捕食螨物种的外部和内部的解剖结构相关的微生物Phytoseiulus螨，Typhlodromips（= 钝绥）swirskii，和香瓜小新绥（= 钝绥）螨-使用光学显微镜，共聚焦激光扫描显微镜和荧光原位杂交（FISH）进行了检查。在体外区域观察到四种微生物形态。这些包括三种微真菌样生物（分别命名为T1，T2和T3）和棒状细菌（T4）。形态型显示在外部区域的独特分布，并且某些微生物仅在一种寄主物种上发现。在所有三个物种中都存在类似微真菌的T1，而仅在柿果假单胞菌和S. swirskii中存在T2和T3 。， 分别。T1和T2微生物在成肌瘤和小腿的腹侧结构中含量最高，最常与Coxae，Coxal褶皱，ventrianal盾和Epigynal盾相关。T3微生物在腿部和背侧特异瘤中含量最高。T4微生物数量较少，附着在黄瓜猪笼草和T. swirskii的附睾盾上。批次之间分布的显着差异表明，在大规模饲养系统中，植物性类植物微生物的时间波动。FISH在消化道内，马氏小管和肛门心房中显示细菌。这些可以帮助吸收排泄产物或维持肠道生理。我们建议一种机制，通过该机制微生物可以传播给后代和整个种群。这项研究旨在提高我们对这个知之甚少的领域的了解，并强调了了解Acari微生物群的必要性。